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Hexokinase 2 Antibody (3D3) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51643

Novus Biologicals, part of Bio-Techne
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NBP1-51643
NBP1-51643-0.025ml

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Rat

Applications

Validated:

ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3D3

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Product Specifications

Immunogen

Purified recombinant fragment of human Hexokinase 2 expressed in E. Coli.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 29137232)..

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

102 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Hexokinase 2 Antibody (3D3) - BSA Free

Western Blot: Hexokinase 2 Antibody (3D3)BSA Free [NBP1-51643]

Western Blot: Hexokinase 2 Antibody (3D3)BSA Free [NBP1-51643]

Western Blot: Hexokinase 2 Antibody (3D3) [NBP1-51643] - Analysis using HK2 mouse mAb against Jurkat (1), Hela (2) and HEK293 (3) cell lysate.
Immunohistochemistry: Hexokinase 2 Antibody (3D3) - BSA Free [NBP1-51643]

Immunohistochemistry: Hexokinase 2 Antibody (3D3) - BSA Free [NBP1-51643]

Immunohistochemistry: Hexokinase 2 Antibody (3D3) [NBP1-51643] - Images of IHC analysis of B7-H3 and HK2 protein expression and hematoxylin and eosin (H&E) staining of CRC (n=126) tissue sections. One representative image is shown. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41419-019-1549-6), licensed under a CC-BY license.
Flow Cytometry: Hexokinase 2 Antibody (3D3) - BSA Free [NBP1-51643]

Flow Cytometry: Hexokinase 2 Antibody (3D3) - BSA Free [NBP1-51643]

Flow Cytometry: Hexokinase 2 Antibody (3D3) [NBP1-51643] - Analysis of K562 cells using HK2 mouse mAb (green) and negative control (purple).

Applications for Hexokinase 2 Antibody (3D3) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Hexokinase 2

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq]

Alternate Names

HK2, HKII, HXK2

Entrez Gene IDs

3099 (Human)

Gene Symbol

HK2

UniProt

Additional Hexokinase 2 Products

Product Documents for Hexokinase 2 Antibody (3D3) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Hexokinase 2 Antibody (3D3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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