Hexokinase 2 Antibody (5Z8C3)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33349
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 5Z8C3
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human Hexokinase 2 (NP_000180.2).
Sequence:
MIASHLLAYFFTELNHDQVQKVDQYLYHMRLSDETLLEISKRFRKEMEKGLGATTHPTAAVKMLPTFVRSTPDGTEHGEFLALDLGGTNFRVLWVKVTDNGLQKVEMENQIYAIPEDIMR
Sequence:
MIASHLLAYFFTELNHDQVQKVDQYLYHMRLSDETLLEISKRFRKEMEKGLGATTHPTAAVKMLPTFVRSTPDGTEHGEFLALDLGGTNFRVLWVKVTDNGLQKVEMENQIYAIPEDIMR
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
102 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Hexokinase 2 Antibody (5Z8C3)
Western Blot: Hexokinase 2 Antibody (5Z8C3) [NBP3-33349] -
Western Blot: Hexokinase 2 Antibody (5Z8C3) [NBP3-33349] - Western blot analysis of various lysates using [KO Validated] Hexokinase 2 Rabbit mAb at 1:25000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western Blot: Hexokinase 2 Antibody (5Z8C3) [NBP3-33349] -
Western Blot: Hexokinase 2 Antibody (5Z8C3) [NBP3-33349] - Western blot analysis of lysates from Mouse skeletal muscle using [KO Validated] Hexokinase 2 Rabbit mAb at 1:25000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Applications for Hexokinase 2 Antibody (5Z8C3)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 ug/mL
Western Blot
1:5000 - 1:30000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Hexokinase 2
Alternate Names
HK2, HKII, HXK2
Gene Symbol
HK2
Additional Hexokinase 2 Products
Product Documents for Hexokinase 2 Antibody (5Z8C3)
Product Specific Notices for Hexokinase 2 Antibody (5Z8C3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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