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Hexokinase 2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44234

Novus Biologicals, part of Bio-Techne
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NBP2-44234

Key Product Details

Species Reactivity

Validated:

Yeast

Cited:

Yeast

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Hexokinase 2 Antibody was produced by repeated immunizations with yeast hexokinase protein. (Uniprot: P04806)

Reactivity Notes

Cross reactivity against Hexokinase 2 from other tissues and species may occur but have not been specifically determined.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Hexokinase [Yeast]

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Hexokinase 2 Antibody

Western Blot: Hexokinase 2 Antibody [NBP2-44234]

Western Blot: Hexokinase 2 Antibody [NBP2-44234]

Western Blot: Hexokinase 2 Antibody [NBP2-44234] - Western Blot of Rabbit Hexokinase 2 2 antibody. Lane 1: Molecular Weight Marker. Lane 2: Hexokinase 2. Primary Antibody: Anti-Hexokinase 2 at 1:1000 overnight in 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:40,000 for 30 mins at RT. Block: Blocking buffer for 30 mins at RT. Expect MW: ~54kDa.
ELISA: Hexokinase 2 Antibody [NBP2-44234]

ELISA: Hexokinase 2 Antibody [NBP2-44234]

ELISA: Hexokinase 2 Antibody [NBP2-44234] - ELISA Results of Rabbit Hexokinase 2 2 antibody tested against purified Hexokinase 2. Each well was coated in duplicate with 1.0 ug of Hexokinase 2 (Yeast). The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer, Goat Anti-Rabbit IgG HRP conjugated and TMB substrate.

Applications for Hexokinase 2 Antibody

Application
Recommended Usage

ELISA

1:3000-1:10000

Immunohistochemistry

1:10-1:500

Western Blot

1:1000-1:5000
Application Notes
This product has been tested by western blotting and for ELISA and is suitable for IHC. Researchers should determine optimal titers for applications that are not stated below.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Background: Hexokinase 2

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq]

Alternate Names

HK2, HKII, HXK2

Gene Symbol

HK2

UniProt

Additional Hexokinase 2 Products

Product Documents for Hexokinase 2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Hexokinase 2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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