Hexokinase 2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44234
Key Product Details
Species Reactivity
Validated:
Yeast
Cited:
Yeast
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Hexokinase 2 Antibody was produced by repeated immunizations with yeast hexokinase protein. (Uniprot: P04806)
Reactivity Notes
Cross reactivity against Hexokinase 2 from other tissues and species may occur but have not been specifically determined.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Hexokinase [Yeast]
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for Hexokinase 2 Antibody
Western Blot: Hexokinase 2 Antibody [NBP2-44234]
Western Blot: Hexokinase 2 Antibody [NBP2-44234] - Western Blot of Rabbit Hexokinase 2 2 antibody. Lane 1: Molecular Weight Marker. Lane 2: Hexokinase 2. Primary Antibody: Anti-Hexokinase 2 at 1:1000 overnight in 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:40,000 for 30 mins at RT. Block: Blocking buffer for 30 mins at RT. Expect MW: ~54kDa.ELISA: Hexokinase 2 Antibody [NBP2-44234]
ELISA: Hexokinase 2 Antibody [NBP2-44234] - ELISA Results of Rabbit Hexokinase 2 2 antibody tested against purified Hexokinase 2. Each well was coated in duplicate with 1.0 ug of Hexokinase 2 (Yeast). The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer, Goat Anti-Rabbit IgG HRP conjugated and TMB substrate.Applications for Hexokinase 2 Antibody
Application
Recommended Usage
ELISA
1:3000-1:10000
Immunohistochemistry
1:10-1:500
Western Blot
1:1000-1:5000
Application Notes
This product has been tested by western blotting and for ELISA and is suitable for IHC. Researchers should determine optimal titers for applications that are not stated below.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: Hexokinase 2
Additional Hexokinase 2 Products
Product Documents for Hexokinase 2 Antibody
Product Specific Notices for Hexokinase 2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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