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Hey L Antibody (1F6)

Novus Biologicals, part of Bio-Techne | Catalog # H00026508-M07

Novus Biologicals, part of Bio-Techne
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H00026508-M07

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # 1F6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

HEYL (NP_055386, 221 a.a. ~ 327 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PLRRATGIILPARRNVLPSRGASSTRRARPLERPATPVPVAPSSRAARSSHIAPLLQSSSPTPPGPTGSAAYVAVPTPNSSSPGPAGRPAGAMLYHSWVSEITEIGA

Reactivity Notes

Other species not tested.

Specificity

HEYL (1F6)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Scientific Data Images for Hey L Antibody (1F6)

Western Blot: Hey L Antibody (1F6) [H00026508-M07]

Western Blot: Hey L Antibody (1F6) [H00026508-M07]

Western Blot: Hey L Antibody (1F6) [H00026508-M07] - Analysis of HEYL expression in HeLa (Cat # L013V1).
Immunocytochemistry/ Immunofluorescence: Hey L Antibody (1F6) [H00026508-M07]

Immunocytochemistry/ Immunofluorescence: Hey L Antibody (1F6) [H00026508-M07]

Immunocytochemistry/Immunofluorescence: Hey L Antibody (1F6) [H00026508-M07] - Analysis of monoclonal antibody to HEYL on HeLa cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: Hey L Antibody (1F6) [H00026508-M07]

Immunohistochemistry-Paraffin: Hey L Antibody (1F6) [H00026508-M07]

Immunohistochemistry-Paraffin: Hey L Antibody (1F6) [H00026508-M07] - Analysis of monoclonal antibody to HEYL on formalin-fixed paraffin-embedded human colon. Antibody concentration 1.5 ug/ml

Applications for Hey L Antibody (1F6)

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluorescence, immunohistochemistry (paraffin) and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Hey L

This gene encodes a member of the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcription factors. The sequence of the encoded protein contains a conserved bHLH and orange domain, but its YRPW motif has diverged from other HESR family members. It is thought to be an effector of Notch signaling and a regulator of cell fate decisions. Alternatively spliced transcript variants have been found, but their biological validity has not been determined.

Alternate Names

BHLHB33, bHLHb33hHeyL, Class B basic helix-loop-helix protein 33, hairy/enhancer-of-split related with YRPW motif 3, hairy/enhancer-of-split related with YRPW motif-like, hairy/enhancer-of-split related with YRPW motif-like protein, Hairy-related transcription factor 3, HEY3, HEY-like protein, hHRT3, HRT3, HRT-3, MGC12623

Entrez Gene IDs

26508 (Human)

Gene Symbol

HEYL

OMIM

609034 (Human)

Additional Hey L Products

Product Documents for Hey L Antibody (1F6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Hey L Antibody (1F6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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