HFE Antibody (1G12)
Novus Biologicals, part of Bio-Techne | Catalog # H00003077-M01
![ELISA: HFE Antibody (1G12) [H00003077-M01]](https://resources.bio-techne.com/images/products/HFE-Antibody-1G12-ELISA-H00003077-M01-img0010.jpg "ELISA: HFE Antibody (1G12) [H00003077-M01]")
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 1G12
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
HFE (NP_000401, 115 a.a. ~ 205 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SHTLQVILGCEMQEDNSTEGYWKYGYDGQDHLEFCPDTLDWRAAEPRAWPTKLEWERHKIRARQNRAYLERDCPAQLQQLLELGRGVLDQQ
Specificity
HFE - hemochromatosis
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for HFE Antibody (1G12)
![Western Blot: HFE Antibody (1G12) [H00003077-M01]](https://resources.bio-techne.com/images/products/HFE-Antibody-1G12-Western-Blot-H00003077-M01-img0009.jpg "Western Blot: HFE Antibody (1G12) [H00003077-M01]")
Western Blot: HFE Antibody (1G12) [H00003077-M01]
Western Blot: HFE Antibody (1G12) [H00003077-M01] - Analysis of HFE expression in transfected 293T cell line by HFE monoclonal antibody (M01), clone 1G12.Lane 1: HFE transfected lysate(40.1 KDa).Lane 2: Non-transfected lysate.![Immunocytochemistry/ Immunofluorescence: HFE Antibody (1G12) [H00003077-M01]](https://resources.bio-techne.com/images/products/HFE-Antibody-1G12-Immunocytochemistry-Immunofluorescence-H00003077-M01-img0006.jpg "Immunocytochemistry/ Immunofluorescence: HFE Antibody (1G12) [H00003077-M01]")
Immunocytochemistry/ Immunofluorescence: HFE Antibody (1G12) [H00003077-M01]
Immunocytochemistry/Immunofluorescence: HFE Antibody (1G12) [H00003077-M01] - Analysis of monoclonal antibody to HFE on HeLa cell. Antibody concentration 10 ug/ml.![Western Blot: HFE Antibody (1G12) [H00003077-M01]](https://resources.bio-techne.com/images/products/HFE-Antibody-1G12-Western-Blot-H00003077-M01-img0008.jpg "Western Blot: HFE Antibody (1G12) [H00003077-M01]")
Western Blot: HFE Antibody (1G12) [H00003077-M01]
Western Blot: HFE Antibody (1G12) [H00003077-M01] - HFE monoclonal antibody (M01), clone 1G12. Analysis of HFE expression in A-431.Applications for HFE Antibody (1G12)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.
Reviewed Applications
Read 2 reviews rated 4.5 using H00003077-M01 in the following applications:
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: HFE
Long Name
Homeostatic Iron Regulator
Alternate Names
HFE1, HLA-H, MVCD7, TFQTL2
Entrez Gene IDs
3077 (Human)
Gene Symbol
HFE
OMIM
176200 (Human)
UniProt
Additional HFE Products
Product Documents for HFE Antibody (1G12)
Product Specific Notices for HFE Antibody (1G12)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...