HGD Antibody (1F1)
Novus Biologicals, part of Bio-Techne | Catalog # H00003081-M11
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunoprecipitation, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 1F1
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
HGD (AAH20792, 1 a.a. ~ 329 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAELKYISGFGNECSSEDPRCPGSLPEGQNNPQVCPYNLYAEQLSGSAFTCPRSTNKRSWLYRILPSVSHKPFESIDEGHVTHNWDEVDPDPNQLRWKPFEIPKASQKKVDFVSGLHTLCGAGDIKSNNGLAIHIFLCNTSMENRCFYNSDGDFLIVPQKGNLLIYTEFGKMLVQPNEICVIQRGMRFSIDVFEETRGYILEVYGVHLELPDLGPIGANGLANPRDFLIPIAWYEDRQVPGGYTVINKYQGKLFAAKQDVSPFNVVAWHGNYTPYKYNLKNFMVINSVAFDHADPSIFTVLTALRRPARSSWHLRGLPMAPWHLCLNHL
Specificity
HGD - homogentisate 1,2-dioxygenase (homogentisate oxidase) (1F1)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for HGD Antibody (1F1)
![Western Blot: HGD Antibody (1F1) [H00003081-M11]](https://resources.bio-techne.com/images/products/HGD-Antibody-1F1-Western-Blot-H00003081-M11-img0003.jpg "Western Blot: HGD Antibody (1F1) [H00003081-M11]")
Western Blot: HGD Antibody (1F1) [H00003081-M11]
Western Blot: HGD Antibody (1F1) [H00003081-M11] - Analysis of HGD expression in transfected 293T cell line by HGD monoclonal antibody (M11), clone 1F1. Lane 1: HGD transfected lysatE (50 KDa). Lane 2: Non-transfected lysate.![Immunoprecipitation: HGD Antibody (1F1) [H00003081-M11]](https://resources.bio-techne.com/images/products/HGD-Antibody-1F1-Immunoprecipitation-H00003081-M11-img0001.jpg "Immunoprecipitation: HGD Antibody (1F1) [H00003081-M11]")
Immunoprecipitation: HGD Antibody (1F1) [H00003081-M11]
Immunoprecipitation: HGD Antibody (1F1) [H00003081-M11] - Analysis of HGD transfected lysate using anti-HGD monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HGD rabbit polyclonal antibody.
Sandwich ELISA: HGD Antibody (1F1) [H00003081-M11] - Detection limit for recombinant GST tagged HGD is 3 ng/ml as a capture antibody.
Applications for HGD Antibody (1F1)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: HGD
Alternate Names
AKU, EC 1.13.11.5, HGOFLJ94126, homogentisate 1,2-dioxygenase, homogentisate 1,2-dioxygenase (homogentisate oxidase), homogentisate oxidase, Homogentisate oxygenase, Homogentisic acid oxidase, Homogentisicase
Entrez Gene IDs
3081 (Human)
Gene Symbol
HGD
UniProt
Additional HGD Products
Product Documents for HGD Antibody (1F1)
Product Specific Notices for HGD Antibody (1F1)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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