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HGD Antibody (7O8Q1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15477

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-15477-100ul
NBP3-15477-20ul

Key Product Details

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7O8Q1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for HGD Antibody (7O8Q1)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 346-445 of human HGD (Q93099). IRGHYEAKQGGFLPGGGSLHSTMTPHGPDADCFEKASKVKLAPERIADGTMAFMFESSLSLAVTKWGLKASRCLDENYHKCWEPLKSHFTPNSRNPAEPN

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HGD Antibody (7O8Q1)

Western Blot: HGD Antibody (7O8Q1) [NBP3-15477]

Western Blot: HGD Antibody (7O8Q1) [NBP3-15477]

Western Blot: HGD Antibody (7O8Q1) [NBP3-15477] - Western blot analysis of extracts of various cell lines, using HGD antibody (NBP3-15477) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Applications for HGD Antibody (7O8Q1)

Application
Recommended Usage

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HGD

Homogentisate 1,2-dioxygenase (HGD) gene mutations are the molecular cause of alkaptonuria, a rare hereditary disorder of the phenylalanine catabolism. The highest expression of HGD is in the prostate, small intestine, colon, and liver. The HGD gene contains 14 exons. Conflicting reports have placed the gene at 3q2, 3q13.3-q21, 3q21-q24, 3q21-q23, or 3q25-q26.

Alternate Names

AKU, EC 1.13.11.5, HGOFLJ94126, homogentisate 1,2-dioxygenase, homogentisate 1,2-dioxygenase (homogentisate oxidase), homogentisate oxidase, Homogentisate oxygenase, Homogentisic acid oxidase, Homogentisicase

Gene Symbol

HGD

Additional HGD Products

Product Documents for HGD Antibody (7O8Q1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HGD Antibody (7O8Q1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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