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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunohistochemistry, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # SBF5 C1.7

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Recombinant Human HGF

Localization

Cytoplasmic

Specificity

SBF5 C1.7 does not cross react with other members of the Skingle-serine protease superfamily such as HGF1/MSP, plasminogen/plasmin. t-PA and u-PA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

82 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HGF Antibody (SBF5 C1.7) - BSA Free

Western Blot: HGF Antibody (SBF5 C1.7)BSA Free [NB100-2696]

Western Blot: HGF Antibody (SBF5 C1.7)BSA Free [NB100-2696]

Western Blot: HGF Antibody (SBF5 C1.7) [NB100-2696] - Expression of miR-10b is elevated in invasive mesenchymal subtype-like U87-2M1 glioma cells. Western blotting analysis of expression of invasiveness-associated proteins in U87-2M1 cells as compared with those in U87 cells. Numbers indicate relative protein expression in U87-2M1 compared with U87 cells after normalization to beta-actin levels. Blots shown are representative of biological triplicates. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/doifinder/10.1038/cddis.2012.134), licensed under a CC-BY license.

Applications for HGF Antibody (SBF5 C1.7) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunohistochemistry

1:10 - 1:500

Western Blot

1:100 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HGF

Hepatocyte growth factor or scatter factor, is a member of the plasminogen related growth factor family. Members are potent effectors of growth, movement and differentiation of epithelial and endothelial cells. HGF/SF may exhibit morphogenesis in adjacent cells. The membrane receptor for HGF/SF is the product of the proto oncogene cmet, a receptor tyrosine kinase.

Long Name

Hepatocyte Growth Factor

Alternate Names

DFNB39, F-TCF, Hepatopoietin A, HGFB, HPTA, SF

Entrez Gene IDs

3082 (Human)

Gene Symbol

HGF

Additional HGF Products

Product Documents for HGF Antibody (SBF5 C1.7) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HGF Antibody (SBF5 C1.7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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