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HIBADH Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82526

Novus Biologicals, part of Bio-Techne
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NBP1-82526

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (93%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NPVPGVMDGVPSANNYQGGFGTTLMAKDLGLAQDSATSTKSPILLGSLAHQIYRMMCAKGYSKKDFSSVFQFLR

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23423614)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HIBADH Antibody

Western Blot: HIBADH Antibody [NBP1-82526]

Western Blot: HIBADH Antibody [NBP1-82526]

Western Blot: HIBADH Antibody [NBP1-82526] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: HIBADH Antibody [NBP1-82526]

Immunohistochemistry-Paraffin: HIBADH Antibody [NBP1-82526]

Immunohistochemistry-Paraffin: HIBADH Antibody [NBP1-82526] - Staining of human kidney.
Immunohistochemistry-Paraffin: HIBADH Antibody [NBP1-82526]

Immunohistochemistry-Paraffin: HIBADH Antibody [NBP1-82526]

Immunohistochemistry-Paraffin: HIBADH Antibody [NBP1-82526] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.

Applications for HIBADH Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HIBADH

3-hydroxyisobutyrate dehydrogenase (3-hydroxy-2-methylpropanoate:NAD(+) oxidoreductase, EC 1.1.1.31) is a dimeric mitochondrial enzyme that catalyzes the NAD(+)-dependent, reversible oxidation of 3-hydroxyisobutyrate, an intermediate of valine catabolism, to methylmalonate semialdehyde.[supplied by OMIM]

Alternate Names

3-hydroxyisobutyrate dehydrogenase, 3-hydroxyisobutyrate dehydrogenase, mitochondrial, EC 1.1.1,3'-hydroxyisobutyrate dehydrogenase, EC 1.1.1.31, MGC40361, NS5ATP1

Gene Symbol

HIBADH

Additional HIBADH Products

Product Documents for HIBADH Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HIBADH Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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