HIC2 Antibody (PCRP-HIC2-1B1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13890

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # PCRP-HIC2-1B1

Concentration

0.2 mg/ml

View all HIC2 Antibodies »

Product Summary for HIC2 Antibody (PCRP-HIC2-1B1)

Immunogen

Recombinant full-length human HIC2protein (Uniprot: Q96JB3)

Localization

Nucleus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

66.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14231)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for HIC2 Antibody (PCRP-HIC2-1B1)

Immunocytochemistry/ Immunofluorescence: HIC2 Antibody (PCRP-HIC2-1B1) [NBP3-13890]

Immunocytochemistry/ Immunofluorescence: HIC2 Antibody (PCRP-HIC2-1B1) [NBP3-13890]

Immunocytochemistry/Immunofluorescence: HIC2 Antibody (PCRP-HIC2-1B1) [NBP3-13890] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using HIC2 Antibody (PCRP-HIC2-1B1) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).
Flow Cytometry: HIC2 Antibody (PCRP-HIC2-1B1) [NBP3-13890]

Flow Cytometry: HIC2 Antibody (PCRP-HIC2-1B1) [NBP3-13890]

Flow Cytometry: HIC2 Antibody (PCRP-HIC2-1B1) [NBP3-13890] - Flow cytometric analysis of PFA-fixed HeLa cells. HIC2 antibody (PCRP-HIC2-1B1) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
Protein Array: HIC2 Antibody (PCRP-HIC2-1B1) [NBP3-13890]

Protein Array: HIC2 Antibody (PCRP-HIC2-1B1) [NBP3-13890]

Protein Array: HIC2 Antibody (PCRP-HIC2-1B1) [NBP3-13890] - Analysis of Protein Array containing more than 19,000 full-length human proteins using HIC2 Antibody (PCRP-HIC2-1B1).

Applications for HIC2 Antibody (PCRP-HIC2-1B1)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunoprecipitation

1-2 ug/100-500 ug protein
Application Notes
ELISA: For coating, order antibody without BSA

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: HIC2

Transcriptional repressor

Alternate Names

HIC1-related gene on chromosome 22 protein, Hic-2, Hic-3, HRG22KIAA1020hic-2, hypermethylated in cancer 2, hypermethylated in cancer 2 protein, ZBTB30hic-3, Zinc finger and BTB domain-containing protein 30, ZNF907

Gene Symbol

HIC2

UniProt

Q96JB3 (Human)

Additional HIC2 Products

Product Documents for HIC2 Antibody (PCRP-HIC2-1B1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HIC2 Antibody (PCRP-HIC2-1B1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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