HIC5/TGFB1I1 Antibody (4B2-D8)

Novus Biologicals, part of Bio-Techne | Catalog # H00007041-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Knockdown Validated, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4B2-D8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HIC5/TGFB1I1 Antibodies »

Product Specifications

Immunogen

TGFB1I1 (AAH32545, 1 a.a. ~ 444 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPRSGAPKERPAEPLTPPPSYGHQPQTGSGESSGASGDKDHLYSTVCKPRSPKPAAPAAPPFSSSSGVLGTGLCELDRLLQELNATQFNITDEIMSQFPSSKVASGEQKEDQSEDKKRPSLPSSPSPGLPKASATSATLELDRLMASLSDFRVQNHLPASGPTQPPVVSSTNEGSPSPPEPTGKGSLDTMLGLLQSDLSRRGVPTQAKGLCGSCNKPIAGQVVTALGRAWHPEHFVCGGCSTALGGSSFFEKDGAPFCPECYFERFSPRCGFCNQPIRHKMVTALGTHWHPEHFCCVSCGEPFGDEGFHEREGRPYCRRDFLQLFAPRCQGCQGPILDNYISALSALWHPDCFVCRECFAPFSGGSFFEHEGRPLCENHFHARRGSLCATCGLPVTGRCVSALGRRFHPDHFTCTFCLRPLTKGSFQERAGKPYCQPCFLKLFG

Specificity

TGFB1I1 - transforming growth factor beta 1 induced transcript 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for HIC5/TGFB1I1 Antibody (4B2-D8)

Western Blot: HIC5/TGFB1I1 Antibody (4B2-D8) [H00007041-M01]

Western Blot: HIC5/TGFB1I1 Antibody (4B2-D8) [H00007041-M01]

Western Blot: HIC5/TGFB1I1 Antibody (4B2-D8) [H00007041-M01] - TGFB1I1 monoclonal antibody (M01), clone 4B2-D8 Analysis of TGFB1I1 expression in Hela.
Western Blot: HIC5/TGFB1I1 Antibody (4B2-D8) [H00007041-M01]

Western Blot: HIC5/TGFB1I1 Antibody (4B2-D8) [H00007041-M01]

Western Blot: HIC5/TGFB1I1 Antibody (4B2-D8) [H00007041-M01] - Analysis of TGFB1I1 over-expressed 293 cell line, cotransfected with TGFB1I1 Validated Chimera RNAi ( Cat # H00007041-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TGFB1I1 monoclonal antibody (M01), clone 4B2-D8 (Cat # H00007041-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: HIC5/TGFB1I1 Antibody (4B2-D8) [H00007041-M01]

Western Blot: HIC5/TGFB1I1 Antibody (4B2-D8) [H00007041-M01]

Western Blot: HIC5/TGFB1I1 Antibody (4B2-D8) [H00007041-M01] - Analysis of TGFB1I1 expression in transfected 293T cell line by TGFB1I1 monoclonal antibody (M01), clone 4B2-D8.Lane 1: TGFB1I1 transfected lysate(47.9 KDa).Lane 2: Non-transfected lysate.
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Applications for HIC5/TGFB1I1 Antibody (4B2-D8)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HIC5/TGFB1I1

The HIC5 gene encodes a coactivator of the androgen receptor, a transcription factor which is activated by androgen and has a key role in male sexual differentiation. The encoded protein is thought to regulate androgen receptor activity and may have a role to

Long Name

Hydrogen Peroxide-inducible Clone 5

Alternate Names

ARA55, TGFB1I1, TSC-5

Entrez Gene IDs

7041 (Human)

Gene Symbol

TGFB1I1

OMIM

602353 (Human)

UniProt

AAH32545 (Human)

Additional HIC5/TGFB1I1 Products

Product Documents for HIC5/TGFB1I1 Antibody (4B2-D8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HIC5/TGFB1I1 Antibody (4B2-D8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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