HID1 Antibody (OTI2F4) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72481

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2F4

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all HID1 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human C17orf28 (NP_085133) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

88.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HID1 Antibody (OTI2F4) - Azide and BSA Free

Western Blot: HID1 Antibody (OTI2F4)Azide and BSA Free [NBP2-72481]

Western Blot: HID1 Antibody (OTI2F4)Azide and BSA Free [NBP2-72481]

Western Blot: HID1 Antibody (OTI2F4) - Azide and BSA Free [NBP2-72481] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C17orf28 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C17orf28.
Immunohistochemistry: HID1 Antibody (OTI2F4) - Azide and BSA Free [NBP2-72481]

Immunohistochemistry: HID1 Antibody (OTI2F4) - Azide and BSA Free [NBP2-72481]

Immunohistochemistry: HID1 Antibody (OTI2F4) - Azide and BSA Free [NBP2-72481] - Staining of paraffin-embedded Human liver tissue using anti-C17orf28 mouse monoclonal antibody.
Flow Cytometry: HID1 Antibody (OTI2F4) - Azide and BSA Free [NBP2-72481]

Flow Cytometry: HID1 Antibody (OTI2F4) - Azide and BSA Free [NBP2-72481]

Flow Cytometry: HID1 Antibody (OTI2F4) - Azide and BSA Free [NBP2-72481] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY C17orf28.
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Applications for HID1 Antibody (OTI2F4) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Western Blot

1:1000-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HID1

HID1 may play an important role in the development of cancers in a broad range of tissues

Alternate Names

C17orf28, chromosome 17 open reading frame 28, DMC1, DMC1FLJ43526, downregulated in multiple cancer 1, Down-regulated in multiple cancers 1, HID-1, hypothetical protein LOC283987

Gene Symbol

HID1

Additional HID1 Products

Product Documents for HID1 Antibody (OTI2F4) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HID1 Antibody (OTI2F4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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