HIF-1 alpha Antibody (2443B) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80764
Recombinant Monoclonal Antibody
![Knockout Validated: HIF-1 alpha Antibody (2443B) - Azide and BSA Free [NBP2-80764]](https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-2443B-Azide-and-BSA-Free-Knockout-Validated-NBP2-80764-img0003.jpg "Knockout Validated: HIF-1 alpha Antibody (2443B) - Azide and BSA Free [NBP2-80764]")
![Western Blot: HIF-1 alpha Antibody (2443B)Azide and BSA Free [NBP2-80764]](https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-2443B-Azide-and-BSA-Free-Western-Blot-NBP2-80764-img0001.jpg "Western Blot: HIF-1 alpha Antibody (2443B)Azide and BSA Free [NBP2-80764]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Knockout/Knockdown, Biological Validation
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Knockout Validated, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2443B
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for HIF-1 alpha Antibody (2443B) - Azide and BSA Free
Immunogen
This recombinant HIF-1 alpha Antibody (2443B) was developed against partial recombinant human HIF-1 alpha protein (amino acids 1-826) [Uniprot# Q16665].
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HIF-1 alpha Antibody (2443B) - Azide and BSA Free
Western Blot: HIF-1 alpha Antibody (2443B)Azide and BSA Free [NBP2-80764]
Western Blot: HIF-1 alpha Antibody (2443B) - Azide and BSA Free [NBP2-80764] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.![Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (2443B) - Azide and BSA Free [NBP2-80764]](https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-2443B-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80764-img0002.jpg "Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (2443B) - Azide and BSA Free [NBP2-80764]")
Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (2443B) - Azide and BSA Free [NBP2-80764]
Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (2443B) - Azide and BSA Free [NBP2-80764] - Immunofluorescent staining of HIF-1 alpha using catalog number NBP2-75977 (clone 2443B). Staining was observed in Hela cells treated with DFO but not in untreated Hela cells. Image from the standard format of this antibody.![Simple Western: HIF-1 alpha Antibody (2443B)Azide and BSA Free [NBP2-80764]](https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-2443B-Azide-and-BSA-Free-Simple-Western-NBP2-80764-img0004.jpg "Simple Western: HIF-1 alpha Antibody (2443B)Azide and BSA Free [NBP2-80764]")
Simple Western: HIF-1 alpha Antibody (2443B)Azide and BSA Free [NBP2-80764]
Simple Western: HIF-1 alpha Antibody (2443B) - Azide and BSA Free [NBP2-80764] - Lane view shows a specific band for HIF-1 alpha in HepG2 +/- DFO cell lysate using 20ug/ml. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 116 KDa. Image from an internal validation. Image from the standard format of this antibody.Applications for HIF-1 alpha Antibody (2443B) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Knockout Validated
Optimal dilutions of this antibody should be experimentally determined.
Simple Western
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Simple western reported by internal validaton.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HIF-1 alpha/HIF1A
HIF-1 or hypoxia inducible factor 1 (predicted molecular weight 93kDa), is a transcription factor commonly referred to as a "master regulator of the hypoxic response" for its central role in the regulation of cellular adaptations to hypoxia. In its active form under hypoxic conditions, HIF-1 is stabilized by the formation of a heterodimer of HIF-1 alpha and ARNT/HIF-1 beta subunits. Nuclear HIF-1 engages p300/CBP for binding to hypoxic response elements (HREs). This process induces transcription and regulation of genes including EPO, VEGF, iNOS2, ANGPT1 and OCT4 (4,5).
Under normoxic conditions, the HIF-1 alpha subunit is rapidly targeted and degraded by the ubiquitin proteasome system. This process is mediated by prolyl hydroxylase domain enzymes (PHDs), which catalyze the hydroxylation of key proline residues (Pro-402 and Pro-564) within the oxygen-dependent degradation domain of HIF-1 alpha. Once hydroxylated, HIF-1 alpha binds the von Hippel-Lindau tumor suppressor protein (pVHL) for subsequent ubiquitination and proteasomal degradation (4). pVHL dependent regulation of HIF-1 alpha plays a role in normal physiology and disease states. Regulation of HIF-1 alpha by pVHL is critical for the suppressive function of FoxP3+ regulatory Tcells (6). Repression of pVHL expression in chronic lymphocytic leukemia (CLL) B cells leads to HIF-1 alpha stabilization and increased VEGF secretion (7).
References
1. Semenza, G. L., Agani, F., Feldser, D., Iyer, N., Kotch, L., Laughner, E., & Yu, A. (2000). Hypoxia, HIF-1, and the pathophysiology of common human diseases. Advances in Experimental Medicine and Biology.
2. Muz, B., de la Puente, P., Azab, F., & Azab, A. K. (2015). The role of hypoxia in cancer progression, angiogenesis, metastasis, and resistance to therapy. Hypoxia. https://doi.org/10.2147/hp.s93413
3. Huang, Y., Lin, D., & Taniguchi, C. M. (2017). Hypoxia inducible factor (HIF) in the tumor microenvironment: friend or foe? Science China Life Sciences. https://doi.org/10.1007/s11427-017-9178-y
4. Koyasu, S., Kobayashi, M., Goto, Y., Hiraoka, M., & Harada, H. (2018). Regulatory mechanisms of hypoxia-inducible factor 1 activity: Two decades of knowledge. Cancer Science. https://doi.org/10.1111/cas.13483
5. Dengler, V. L., Galbraith, M. D., & Espinosa, J. M. (2014). Transcriptional regulation by hypoxia inducible factors. Critical Reviews in Biochemistry and Molecular Biology. https://doi.org/10.3109/10409238.2013.838205
6. Lee, J. H., Elly, C., Park, Y., & Liu, Y. C. (2015). E3Ubiquitin Ligase VHL Regulates Hypoxia-Inducible Factor-1 alpha to Maintain Regulatory T Cell Stability and Suppressive Capacity. Immunity. https://doi.org/10.1016/j.immuni.2015.05.016
7. Ghosh, A. K., Shanafelt, T. D., Cimmino, A., Taccioli, C., Volinia, S., Liu, C. G., ... Kay, N. E. (2009). Aberrant regulation of pVHL levels by microRNA promotes the HIF/VEGF axis in CLL B cells. Blood. https://doi.org/10.1182/blood-2008-10-185686
Long Name
Hypoxia Inducible Factor 1 Subunit Alpha
Alternate Names
BHLHE78, HIF 1A, HIF-1a, HIF1 alpha, HIF1A, MOP1, PASD8
Gene Symbol
HIF1A
Additional HIF-1 alpha/HIF1A Products
Product Documents for HIF-1 alpha Antibody (2443B) - Azide and BSA Free
Product Specific Notices for HIF-1 alpha Antibody (2443B) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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