HIF-2 alpha/EPAS1 Antibody (ep190b) [HRP]
Novus Biologicals, part of Bio-Techne | Catalog # NB100-132H
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Bovine, Hamster
Cited:
Human
Applications
Validated:
Chromatin Immunoprecipitation (ChIP), ELISA, Flow Cytometry, Gel Super Shift Assays, Gel Supershift Assay, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, In vivo assay, Knockdown Validated, Simple Western, Western Blot
Cited:
IF/IHC
Label
HRP
Antibody Source
Monoclonal Mouse IgG1 Clone # ep190b
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
The immunogen recognized by this HIF-2 alpha/EPAS1 Antibody (ep190b) maps to a region between amino acids 535-631. [UniProt# Q99814]
Reactivity Notes
Ability to use HIF-2 alpha/EPAS1 Antibody (ep190b) in mouse is mixed with some positive and some negative results. Use in Bovine reported in scientific literature (PMID:32054096).
Specificity
This HIF-2 alpha/EPAS1 Antibody (ep190b) is specific for HIF-2 alpha/EPAS1, and does not cross-react with HIF-1 alpha.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for HIF-2 alpha/EPAS1 Antibody (ep190b) [HRP]
Western Blot: HIF-2 alpha/EPAS1 Antibody (ep190b) [HRP] [NB100-132H] - Analysis of HIF-2 alpha stabilization over time in HOS cells following exposure to hypoxia. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23785417/) licensed under a CC-BY license.
Western Blot: HIF-2 alpha/EPAS1 Antibody (ep190b) [HRP] [NB100-132H] - Analysis of HIF-2 alpha stabilization over time in 791T cells following exposure to hypoxia. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23785417/) licensed under a CC-BY license.
![HIF-2 alpha/EPAS1 Antibody (ep190b) [HRP]](https://resources.bio-techne.com/images/products/nb100-132h_mouse-monoclonal-hif-2-alpha-epas1-antibody-ep190b-hrp-310202415535111.jpg "Western Blot: HIF-2 alpha/EPAS1 Antibody (ep190b) [HRP] [NB100-132H] -")
Western Blot: HIF-2 alpha/EPAS1 Antibody (ep190b) [HRP] [NB100-132H] -
Western Blot: HIF-2 alpha/EPAS1 Antibody (ep190b) [HRP] [NB100-132H] - Regulation of HIF-1 expression in osteosarcoma cells.A, Western blots showing the time course of HIF-1 alpha & HIF-2 alpha stabilisation & protein levels of the HIF-1 transcriptional target carbonic anhydrase IX (CA IX) in 791T, HOS & U2OS cells after exposure to hypoxia. GAPDH is a loading control. Data are representative of 3 independent experiments. B, Graphs show 2(− delta deltaCT) where CT is the cross-threshold & represents the change in Glut-1 mRNA expression with time in 791T, HOS & U2OS cells in hypoxia relative to normoxia, where 1 would be equivalent expression in normoxia & hypoxia & greater than 1 represents an increase in hypoxia relative to normoxia. Data are the mean ± SEM of 2 independent experiments. C, VEGF-A levels in the supernatant of HOS & U2OS cells detected by enzyme-linked immunosorbent assay after 24 hours in normoxia or hypoxia, normalised to cell number. Data are the mean ± SEM of 3 independent experiments. * indicates p<0.05 as determined by the 2-tailed student t-test. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23785417), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for HIF-2 alpha/EPAS1 Antibody (ep190b) [HRP]
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
Optimal dilutions of this antibody should be experimentally determined.
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Gel Super Shift Assays
Optimal dilutions of this antibody should be experimentally determined.
Gel Supershift Assay
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Frozen
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
In vivo assay
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Simple Western
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: HIF-2 alpha/EPAS1
HIF-1 or hypoxia inducible factor 1, is a transcription factor commonly referred to as a "master regulator of the hypoxic response" for its central role in the regulation of cellular adaptations to hypoxia. Similarly, HIF-2 alpha plays a role in cellular responses to hypoxia, but whereas HIF-1 alpha is ubiquitously expressed, HIF-2 alpha is predominantly expressed in the vascular endothelium at embryonic stages and after birth in select cells and tissue types (e.g., fibroblasts, hepatocytes and myocytes at 96kDa) (4). Following a similar mechanism to HIF-1 alpha, HIF-2 alpha is stabilized under hypoxic conditions by the formation of a heterodimer with an ARNT/HIF-1 beta subunit. Stable HIF-2 alpha-ARNT/HIF-1 beta heterodimers engage p300/CBP in the nucleus for binding to hypoxic response elements (HREs), inducing transcription, and thus regulation of genes (e.g., EPO, VEGFA). HIF-1 predominantly transactivates genes involved in glycolytic control and pro- apoptotic genes (e.g., LDHA and BNIP3), and HIF-2 regulates the expression of genes involved in invasion and stemness (e.g., MMP2, and OCT4). Common gene targets for HIF-1 and HIF-2 include VEGFA and GLUT1 (5).
The HIF-2 alpha subunit is rapidly targeted and degraded by the ubiquitin proteasome system under normoxic conditions. This process is mediated by oxygen-sensing enzymes, prolyl hydroxylase domain enzymes (PHDs), which catalyze the hydroxylation of key proline residues (Pro-405 and Pro-531) within the oxygen-dependent degradation domain of HIF-2 alpha (5). Once hydroxylated, HIF-2 alpha binds the von Hippel-Lindau tumor suppressor protein (pVHL) for subsequent ubiquitination and proteasomal degradation (5,6).
References
1. Semenza, G. L., Agani, F., Feldser, D., Iyer, N., Kotch, L., Laughner, E., & Yu, A. (2000). Hypoxia, HIF-1, and the pathophysiology of common human diseases. Advances in Experimental Medicine and Biology.
2.Muz, B., de la Puente, P., Azab, F., & Azab, A. K. (2015). The role of hypoxia in cancer progression, angiogenesis, metastasis, and resistance to therapy. Hypoxia. https://doi.org/10.2147/hp.s93413
3. Huang, Y., Lin, D., & Taniguchi, C. M. (2017). Hypoxia inducible factor (HIF) in the tumor microenvironment: friend or foe? Science China Life Sciences. https://doi.org/10.1007/s11427-017-9178-y
4. Hu, C.-J., Wang, L.-Y., Chodosh, L. A., Keith, B., & Simon, M. C. (2003). Differential Roles of Hypoxia-Inducible Factor 1 (HIF-1) and HIF-2 in Hypoxic Gene Regulation. Molecular and Cellular Biology. https://doi.org/10.1128/mcb.23.24.9361-9374.2003
5. Koh, M. Y., & Powis, G. (2012). Passing the baton: The HIF switch. Trends in Biochemical Sciences. https://doi.org/10.1016/j.tibs.2012.06.004
6. Koyasu, S., Kobayashi, M., Goto, Y., Hiraoka, M., & Harada, H. (2018). Regulatory mechanisms of hypoxia-inducible factor 1 activity: Two decades of knowledge. Cancer Science. https://doi.org/10.1111/cas.13483
Long Name
Hypoxia-inducible Transcription Factor 2 alpha
Alternate Names
EPAS1, HIF 2A, HIF2 alpha, HIF2A, HLF, MOP2
Gene Symbol
EPAS1
Additional HIF-2 alpha/EPAS1 Products
Product Documents for HIF-2 alpha/EPAS1 Antibody (ep190b) [HRP]
Product Specific Notices for HIF-2 alpha/EPAS1 Antibody (ep190b) [HRP]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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