Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Rabbit (100%). Backed by our 100% Guarantee.

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all HINT1 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 17 amino acid synthetic peptide near the center of the human HINT1. The immunogen is located within the last 50 amino acids of HINT1. Amino Acid Squence: NKGYRMVVNEGSDGGQS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HINT1 Antibody - BSA Free

Western Blot: HINT1 AntibodyBSA Free [NBP1-76944]

Western Blot: HINT1 AntibodyBSA Free [NBP1-76944]

Western Blot: HINT1 Antibody [NBP1-76944] - Jurkat lysate with HINT1 antibody at (A) 1 and (B) 2 ug/mL.
HINT1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: HINT1 Antibody - BSA Free [NBP1-76944] -

Immunocytochemistry/ Immunofluorescence: HINT1 Antibody - BSA Free [NBP1-76944] - Immunocytochemistry of HINT1 in Jurkat cells with HINT1 antibody at 2.5 ug/mL.

Applications for HINT1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

2.5 ug/mL

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HINT1

Histidine triad nucleotide-binding protein 1 (HINT1) is a member of the histidine triad (HIT) protein family, a group of small nucleotide-binding and -hydrolyzing proteins. HINT1 interacts with several diverse proteins and has been suggested to have tumor suppressive activities. HINT1 catalyzes the hydrolysis of adenosine 5'-monophoramidate substrates such as AMP-morpholidate, but its enzymatic function does not appear to play a role in its tumor suppression. Recent experiments demonstrate that HINT1 forms a complex with POSH and JNK in vivo, inhibiting AP-1 activity and the phosphorylation of c-Jun, and this action could contribute to the tumor suppressor activity of HINT1. Other studies raise the possibility of HINT1 as a candidate gene for schizophrenia.

Alternate Names

Adenosine 5'-monophosphoramidase, EC 3.-, HINT, histidine triad nucleotide binding protein 1, histidine triad nucleotide-binding protein, histidine triad nucleotide-binding protein 1, PKCI1, PKCI-1FLJ30414, PRKCNH1FLJ32340, Protein kinase C inhibitor 1, Protein kinase C-interacting protein 1

Gene Symbol

HINT1

UniProt

NP_005331 (Human)

Additional HINT1 Products

Product Documents for HINT1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HINT1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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