His Tag Antibody (AD1.1.10) - Non-Recombinant Monoclonal

Novus Biologicals, part of Bio-Techne | Catalog # NB100-64768

Novus Biologicals, part of Bio-Techne
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NB100-64768
NB100-64768SS
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Key Product Details

Species Reactivity

Validated:

Epitope Tag

Cited:

Human, Mouse

Applications

Validated:

ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, In vitro assay, Simple Western, Western Blot

Cited:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # AD1.1.10

Format

Non-Recombinant Monoclonal

Concentration

1.0 mg/ml

View all His Tag Antibodies »

Product Specifications

Immunogen

This His Tag Antibody (AD1.1.10) - Non-Recombinant Monoclonal was developed against PAX6 transcription factor linked to histidine tag.

Reactivity Notes

6x His Tag specific

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for His Tag Antibody (AD1.1.10) - Non-Recombinant Monoclonal

Simple Western: His Tag Antibody (AD1.1.10)Non-Recombinant Monoclonal [NB100-64768]

Simple Western: His Tag Antibody (AD1.1.10)Non-Recombinant Monoclonal [NB100-64768]

Simple Western: His Tag Antibody (AD1.1.10) [NB100-64768] - Image shows a specific band for 6-His Epitope Tag in 0.1 mg/mL of rhEDG6 CHO S Tx lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Western Blot: His Tag Antibody (AD1.1.10)Non-Recombinant Monoclonal [NB100-64768]

Western Blot: His Tag Antibody (AD1.1.10)Non-Recombinant Monoclonal [NB100-64768]

Western Blot: His Tag Antibody (AD1.1.10) [NB100-64768] - Analysis using the Biotin conjugate of NB100-64768. Detection of HIS tagged protein probed with mouse anti histidine tag : Biotin and visualised with Streptavidin : HRP.
Flow Cytometry: His Tag Antibody (AD1.1.10) - Non-Recombinant Monoclonal [NB100-64768]

Flow Cytometry: His Tag Antibody (AD1.1.10) - Non-Recombinant Monoclonal [NB100-64768]

Flow Cytometry: His Tag Antibody (AD1.1.10) [NB100-64768] - Analysis using the FITC conjugate of NB100-64768. Staining of Human anti-human CD5 (HuCAL dHLX antibody with HIS tag) human peripheral blood lymphocytes, visualised with FITC conjugated Mouse anti-HIS tag antibody
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Applications for His Tag Antibody (AD1.1.10) - Non-Recombinant Monoclonal

Application
Recommended Usage

ELISA

1:100 - 1:500

Flow Cytometry

1:10 - 1:50

Immunohistochemistry

1:100 - 1:300

Immunohistochemistry-Frozen

1:100 - 1:300

Immunoprecipitation

1:10 - 1:500

Simple Western

1:250

Western Blot

1:1000
Application Notes
This antibody is routinely tested in WB using Histidine-tagged recombinant proteins. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using NB100-64768 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Non-Recombinant Monoclonal

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: His Tag

A his-tag (also known as histidine tag or polyhistidine tag) is a common epitope tag that typically consists of at least 6 histidine residues fused to either the carboxyl (C-) or amino (N-) terminus of a targeted recombinant protein to facilitate its purification and detection (1). The most common his-tag is the hexahistidine (His6/6-His) tag which has a theoretical molecular weight of 0.8kda (1). The histidine residues readily interact with transition metal ions such as Co2+, Ni2+, Cu2+, and Zn2+, making immobilized metal-affinity chromatography (IMAC) the preferred technique for his-tag purification (1, 2). Metal ions are immobilized and bound to by His-tags in the IMAC column via the histidine imidazole ring. The tagged protein can be eluted off the column by washing with buffers containing a low concentration of imidazole (1, 2). Due to its relatively small size, low immunogenicity, versatility under denaturing conditions, and minimal interference with the structure and function of proteins, the his-tag is one of the most widely used tags for protein purification (1-3).

References

1. Malhotra, A. (2009). Tagging for protein expression. Methods in Enzymology, Guide to Protein Purification, 2nd Edition, 463, 239-258. https://doi.org/10.1016/s0076-6879(09)63016-0

2. Terpe, K. (2003). Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Applied Microbiology and Biotechnology, 60(5), 523-533. https://doi.org/10.1007/s00253-002-1158-6

3. Booth, W. T., Schlachter, C. R., Pote, S., Ussin, N., Mank, N. J., Klapper, V., ... Chruszcz, M. (2018). Impact of an N-terminal polyhistidine tag on protein thermal stability. ACS Omega, 3(1), 760-768. https://doi.org/10.1021/acsomega.7b01598

Long Name

Histidine Tag

Alternate Names

polyHistidine

Additional His Tag Products

Product Documents for His Tag Antibody (AD1.1.10) - Non-Recombinant Monoclonal

Product Datasheets

Certificate of Analysis

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Product Specific Notices for His Tag Antibody (AD1.1.10) - Non-Recombinant Monoclonal

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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