His Tag Antibody (RMH01) - Chimeric

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61478

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Epitope Tag

Applications

ELISA, Sandwich ELISA Capture, Sandwich ELISA Detection, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # RMH01

Concentration

1 mg/ml

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Product Specifications

Immunogen

This His Tag Antibody (RMH01) was developed against a mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis Tag at the C-terminus. This antibody is a Rabbit-Human Chimeric antibody, made by fusion of the antigen binding region (variable domains of the heavy and light chains, VH and VL) of NBP2-61482, with the constant domain of Human IgG1. The antibody is recognized only by an anti-Human IgG secondary antibody.

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Scientific Data Images for His Tag Antibody (RMH01) - Chimeric

Western Blot: His Tag Antibody (RMH01) [NBP2-61478] -

Western Blot: His Tag Antibody (RMH01) [NBP2-61478] - Western blot of 293T cells transfected (+) or untransfected (-) with a DNA construct encoding His-Tag Histone H3 (G34W) protein, using NBP2-61478 at 0.2 ug/mL, followed by a HRP conjugated anti-Human IgG secondary antibody.
Simple Western: His Tag Antibody (RMH01)Chimeric [NBP2-61478]

Simple Western: His Tag Antibody (RMH01)Chimeric [NBP2-61478]

Simple Western: His Tag Antibody (RMH01) [NBP2-61478] - Human purified protein.

ELISA: His Tag Antibody (RMH01) [NBP2-61478] -

ELISA: His Tag Antibody (RMH01) [NBP2-61478] - A titer ELISA performed on purified HisTag-PCSK9 recombinant protein. A serial dilution of NBP2-61478 was used as the primary antibody. An alkaline phosphatase conjugated anti-Human IgG as the secondary antibody.
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Applications for His Tag Antibody (RMH01) - Chimeric

Application
Recommended Usage

Sandwich ELISA Capture

0.1 - 2 ug/ml

Sandwich ELISA Detection

0.01 - 0.5 ug/ml

Western Blot

0.1 ug/ml - 1 ug/ml

Reviewed Applications

Read 1 review rated 5 using NBP2-61478 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50% Glycerol/PBS, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: His Tag

A his-tag (also known as histidine tag or polyhistidine tag) is a common epitope tag that typically consists of at least 6 histidine residues fused to either the carboxyl (C-) or amino (N-) terminus of a targeted recombinant protein to facilitate its purification and detection (1). The most common his-tag is the hexahistidine (His6/6-His) tag which has a theoretical molecular weight of 0.8kda (1). The histidine residues readily interact with transition metal ions such as Co2+, Ni2+, Cu2+, and Zn2+, making immobilized metal-affinity chromatography (IMAC) the preferred technique for his-tag purification (1, 2). Metal ions are immobilized and bound to by His-tags in the IMAC column via the histidine imidazole ring. The tagged protein can be eluted off the column by washing with buffers containing a low concentration of imidazole (1, 2). Due to its relatively small size, low immunogenicity, versatility under denaturing conditions, and minimal interference with the structure and function of proteins, the his-tag is one of the most widely used tags for protein purification (1-3).

References

1. Malhotra, A. (2009). Tagging for protein expression. Methods in Enzymology, Guide to Protein Purification, 2nd Edition, 463, 239-258. https://doi.org/10.1016/s0076-6879(09)63016-0

2. Terpe, K. (2003). Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Applied Microbiology and Biotechnology, 60(5), 523-533. https://doi.org/10.1007/s00253-002-1158-6

3. Booth, W. T., Schlachter, C. R., Pote, S., Ussin, N., Mank, N. J., Klapper, V., ... Chruszcz, M. (2018). Impact of an N-terminal polyhistidine tag on protein thermal stability. ACS Omega, 3(1), 760-768. https://doi.org/10.1021/acsomega.7b01598

Long Name

Histidine Tag

Alternate Names

polyHistidine

Additional His Tag Products

Product Documents for His Tag Antibody (RMH01) - Chimeric

Product Datasheets

Certificate of Analysis

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Product Specific Notices for His Tag Antibody (RMH01) - Chimeric

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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