HIST1H3A [p Ser10] Antibody (921)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43620
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 921
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for HIST1H3A [p Ser10] Antibody (921)
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Ser10 of human HIST1H3A. The exact sequence is proprietary.
Modification
p Ser10
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for HIST1H3A [p Ser10] Antibody (921)
Immunocytochemistry/Immunofluorescence: HIST1H3A [p Ser10] Antibody (921) [NBP2-43620] - Analysis of Samples: HCT116 cells mock (left) and treated with 30 uM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: p21 Cip1 protein stained by p21 Cip1 antibody. Red: Histone H3S10ph (phospho Ser10) protein stained by Histone H3S10ph (phospho Ser10) antibody [921]. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: HIST1H3A [p Ser10] Antibody (921) [NBP2-43620] - Mouse brain. Histone H3S10ph (phospho Ser10) stained by Histone H3S10ph (phospho Ser10) antibody [921] diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Applications for HIST1H3A [p Ser10] Antibody (921)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: HIST1H3A
Alternate Names
H3 histone family, member A, H3/A, H3FAHIST1H3E, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, histone 1, H3a, histone cluster 1, H3a, histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l
Gene Symbol
H3C1
Additional HIST1H3A Products
Product Documents for HIST1H3A [p Ser10] Antibody (921)
Product Specific Notices for HIST1H3A [p Ser10] Antibody (921)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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