HIST1H3A Antibody (9G8)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26231
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG Clone # 9G8
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for HIST1H3A Antibody (9G8)
Immunogen
Recombinant Human HIST1H3A protein [UniProt P68431]
Clonality
Monoclonal
Host
Mouse
Isotype
IgG
Scientific Data Images for HIST1H3A Antibody (9G8)
Immunohistochemistry: HIST1H3A Antibody (9G8) [NBP3-26231] -
Immunohistochemistry: HIST1H3A Antibody (9G8) [NBP3-26231] - Image of HIST1H3A Antibody (9G8) diluted at 1:300 and staining in paraffin-embedded human salivary gland tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
Western Blot: HIST1H3A Antibody (9G8) [NBP3-26231] -
Western Blot: HIST1H3A Antibody (9G8) [NBP3-26231] - Positive Western Blot detected in: MCF7 whole cell lysate, JK whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate.All lanes: HIST1H3A Antibody at 1: 1000
Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution.
Predicted band size: 16 kDa
Observed band size: 16 kDa
Immunocytochemistry/Immunofluorescence: HIST1H3A Antibody (9G8) [NBP3-26231] -
Immunocytochemistry/Immunofluorescence: HIST1H3A Antibody (9G8) [NBP3-26231] - Staining of HepG2 cell with HIST1H3A Antibody (9G8) at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated Goat Anti-Mouse IgG (H+L).Applications for HIST1H3A Antibody (9G8)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
50% Glycerol, 0.01M PBS, PH 7.4
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: HIST1H3A
Alternate Names
H3 histone family, member A, H3/A, H3FAHIST1H3E, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, histone 1, H3a, histone cluster 1, H3a, histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l
Gene Symbol
H3C1
Additional HIST1H3A Products
Product Specific Notices for HIST1H3A Antibody (9G8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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