HIST1H3A Antibody (9G8)

Immunohistochemistry: HIST1H3A Antibody (9G8) [NBP3-26231] -

Immunohistochemistry: HIST1H3A Antibody (9G8) [NBP3-26231] - Image of HIST1H3A Antibody (9G8) diluted at 1:300 and staining in paraffin-embedded human salivary gland tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.

Western Blot: HIST1H3A Antibody (9G8) [NBP3-26231] -

Western Blot: HIST1H3A Antibody (9G8) [NBP3-26231] - Positive Western Blot detected in: MCF7 whole cell lysate, JK whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate.
All lanes: HIST1H3A Antibody at 1: 1000
Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution.
Predicted band size: 16 kDa
Observed band size: 16 kDa

Immunocytochemistry/Immunofluorescence: HIST1H3A Antibody (9G8) [NBP3-26231] -

Immunocytochemistry/Immunofluorescence: HIST1H3A Antibody (9G8) [NBP3-26231] - Staining of HepG2 cell with HIST1H3A Antibody (9G8) at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated Goat Anti-Mouse IgG (H+L).