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Histamine Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45266

Novus Biologicals, part of Bio-Techne
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NBP2-45266-0.2ml
NBP2-45266-0.1ml
NBP2-45266-0.025ml

Key Product Details

Species Reactivity

Validated:

Human, All Species

Cited:

Human

Applications

Validated:

Dot Blot, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Histamine conjugated to succinylated KLH.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 30758643).

Specificity

The product specifically stains histamine-containing cells in paraformaldehyde/carbodiimide-fixed or paraformaldehyde-fixed, paraffin-embedded sections of rat stomach (endocrine cells and mast cells).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histamine Antibody

Immunocytochemistry: Histamine Antibody [NBP2-45266]

Immunocytochemistry: Histamine Antibody [NBP2-45266]

Immunocytochemistry: Histamine Antibody [NBP2-45266] - Formalin fixed, paraffin-embedded Rat stomach tissue sections were stained with 1:100 Anti-Histamine antibody produced in Rabbit, followed by 25 ug/mL Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in Goat and by 25 ug/mL ExtrAvidin-Peroxidase.
Immunohistochemistry: Histamine Antibody [NBP2-45266]

Immunohistochemistry: Histamine Antibody [NBP2-45266]

Immunohistochemistry: Histamine Antibody [NBP2-45266] - Formalin fixed, paraffin-embedded Rat stomach tissue sections were stained with 1:200 Anti-Histamine antibody produced in Rabbit (Cat. No. H7403), followed by 25 ug/mL Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in Goat and by 25 ug/mL ExtrAvidin-Peroxidase.

Applications for Histamine Antibody

Application
Recommended Usage

Dot Blot

1:14000

Immunohistochemistry-Paraffin

1:100 - 1:200
Application Notes
Dot Blot: 1:14,000 using histamine-BSA.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

10mM PBS (pH 7.4) and 1.0% BSA

Preservative

0.9% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histamine

Histamine is located in mast cells, endocrine cells of the gut, blood cells and in some cells of the peripheral and central nervous system. Histamine is a potent vasodilator when secreted by mast cells found in various tissues as a result of allergic hypersensitivity or inflammation. Histamine causes running nose, sneezing and itching, and narrows the airways in the lungs. In the central nervous system, Histamine is putative neurotransmitter. In the brain, its highest content has been found in the hypothalamus and in certain areas of the mesencephalon.

Alternate Names

2 (4 imidazolyl)ethylamine, 4 (2 aminoethyl) 1 3 diazole

Additional Histamine Products

Product Documents for Histamine Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Histamine Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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