Histamine Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13403
Key Product Details
Species Reactivity
Validated:
Rat
Applications
Dot Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for Histamine Antibody
Immunogen
The immunogen used to generate this antibody corresponds to Histamine
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Centrifuge briefly prior to opening.
Scientific Data Images for Histamine Antibody
![Immunocytochemistry/ Immunofluorescence: Histamine Antibody [NBP3-13403]](https://resources.bio-techne.com/images/products/Histamine-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13403-img0003.jpg "Immunocytochemistry/ Immunofluorescence: Histamine Antibody [NBP3-13403]")
Immunocytochemistry/ Immunofluorescence: Histamine Antibody [NBP3-13403]
Immunocytochemistry/Immunofluorescence: Histamine Antibody [NBP3-13403] - Histamine antibody detects Histamine protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histamine stained by Histamine antibody (NBP3-13403) diluted at 1:500. Red: Tau, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI.
Dot Blot: Histamine Antibody [NBP3-13403] - Dot blot analysis of 100 ng BSA-conjugated Histamine, using Histamine antibody (NBP3-13403) at 1:4000, 1:8000, 1:16000 and 1:32000.
![ELISA: Histamine Antibody [NBP3-13403]](https://resources.bio-techne.com/images/products/Histamine-Antibody-ELISA-NBP3-13403-img0002.jpg "ELISA: Histamine Antibody [NBP3-13403]")
ELISA: Histamine Antibody [NBP3-13403]
ELISA: Histamine Antibody [NBP3-13403] - ELISA plate is coated with 100 uL of Histamine-carrier protein conjugate at 10 ug/mL. The coated ligand is detected with Histamine antibody (NBP3-13403) at concentrations ranging from 440 ng/mL down to 1.6 ng/mL. Rabbit IgG antibody (HRP) (NBP2-19301) is diluted at 1:2,000 and used to detect the primary antibody.Applications for Histamine Antibody
Application
Recommended Usage
Dot Blot
Optimal dilutions of this antibody should be experimentally determined.
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Histamine
Alternate Names
2 (4 imidazolyl)ethylamine, 4 (2 aminoethyl) 1 3 diazole
Additional Histamine Products
Product Specific Notices for Histamine Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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