Histamine H1R Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-06039

Novus Biologicals, part of Bio-Techne
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NBP1-06039
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Key Product Details

Species Reactivity

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Simple Western, Western Blot

Cited:

Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Histamine H1 R Antibodies »

Product Specifications

Immunogen

Peptide with sequence QQTSVRREDKCETD corresponding to internal region according to NP_000852.1.

Epitope

QQTSVRREDKCETD

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Histamine H1R Antibody

Western Blot: Histamine H1R Antibody [NBP1-06039]

Western Blot: Histamine H1R Antibody [NBP1-06039]

Western Blot: Histamine H1 R Antibody [NBP1-06039] - (0.5ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Histamine H1R Antibody [NBP1-06039]

Immunohistochemistry-Paraffin: Histamine H1R Antibody [NBP1-06039]

Immunohistochemistry-Paraffin: Histamine H1 R Antibody [NBP1-06039] - (4ug/ml) staining of paraffin embedded Human Lung. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

Applications for Histamine H1R Antibody

Application
Recommended Usage

Immunohistochemistry

2-4ug/ml

Immunohistochemistry-Paraffin

2-4ug/ml

Peptide ELISA

Detection limit 1:16000

Western Blot

0.5 - 1.5 ug/ml
Application Notes
WB: Approx. 55 kDa band observed in human heart lysates (calculated MW of 55.8 kDa band according to NP_000852.1).
HC: In paraffin embedded Human Lung shows membranous staining of epitheilial cells in tertiar bronchus and in alveoli.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Histamine H1 R

Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. This gene was thought to be intronless until recently. The protein encoded by this gene is an integral membrane protein and belongs to the G protein-coupled receptor superfamily. It mediates the contraction of smooth muscles, the increase in capillary permeability due to contraction of terminal venules, the release of catecholamine from adrenal medulla, and neurotransmission in the central nervous system. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Long Name

Histamine H1 Receptor

Alternate Names

H1-R, Histamine H1R, HRH1

Entrez Gene IDs

3269 (Human)

Gene Symbol

HRH1

UniProt

NP_000852.1 (Human)

Additional Histamine H1 R Products

Product Documents for Histamine H1R Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Histamine H1R Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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