Histone Deacetylase 2/HDAC2 Antibody (9H4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26239
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 9H4
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human Histone Deacetylase 2/HDAC2 [UniProt Q92769]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Histone Deacetylase 2/HDAC2 Antibody (9H4)
Immunohistochemistry: Histone Deacetylase 2/HDAC2 Antibody (9H4) [NBP3-26239] -
Immunohistochemistry: Histone Deacetylase 2/HDAC2 Antibody (9H4) [NBP3-26239] - Image of Histone Deacetylase 2/HDAC2 Antibody (9H4) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunohistochemistry: Histone Deacetylase 2/HDAC2 Antibody (9H4) [NBP3-26239] -
Immunohistochemistry: Histone Deacetylase 2/HDAC2 Antibody (9H4) [NBP3-26239] - Image of Histone Deacetylase 2/HDAC2 Antibody (9H4) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot: Histone Deacetylase 2/HDAC2 Antibody (9H4) [NBP3-26239] -
Western Blot: Histone Deacetylase 2/HDAC2 Antibody (9H4) [NBP3-26239] - Positive Western Blot detected in: MCF-7 whole cell lysate, K562 whole cell lysate.All lanes: Histone Deacetylase 2/HDAC2 Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 56, 52 kDa
Observed band size: 60 kDa
Applications for Histone Deacetylase 2/HDAC2 Antibody (9H4)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:20-1:200
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: Histone Deacetylase 2/HDAC2
Long Name
Histone Deacetylase 2
Alternate Names
HDAC2, YAF1
Gene Symbol
HDAC2
Additional Histone Deacetylase 2/HDAC2 Products
Product Documents for Histone Deacetylase 2/HDAC2 Antibody (9H4)
Product Specific Notices for Histone Deacetylase 2/HDAC2 Antibody (9H4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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