Histone Deacetylase 2/HDAC2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-44095
![Western Blot: Histone Deacetylase 2/HDAC2 Antibody [NBP1-44095]](https://resources.bio-techne.com/images/products/Histone-Deacetylase-2-HDAC2-Antibody-Western-Blot-NBP1-44095-img0003.jpg "Western Blot: Histone Deacetylase 2/HDAC2 Antibody [NBP1-44095]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Primate
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Histone Deacetylase 2/Histone Deacetylase 2/HDAC2 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the near C-terminal region of human Histone Deacetylase 2/HDAC2. (Uniprot: Q92769)
Reactivity Notes
A BLAST analysis was used to suggest cross-reactivity with Human, Mouse, Rat, and Primate based on 100% sequence homology. Cross-reactivity with HDAC2 from other sources has not been determined.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for Histone Deacetylase 2/HDAC2 Antibody
Western Blot: Histone Deacetylase 2/HDAC2 Antibody [NBP1-44095]
Western Blot: Histone Deacetylase 2/HDAC2 Antibody [NBP1-44095] - Lane 1: mouse brain extract. Lane 2: mouse brain extract blocked with peptide. Load: 4 ug per lane. Primary antibody: HDAC2 antibody at 1ug/mL for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 59 kDa for HDAC2.Applications for Histone Deacetylase 2/HDAC2 Antibody
Application
Recommended Usage
ELISA
1:20000-1:60000
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Immunohistochemistry
1:100-1:500
Western Blot
1 ug/ml
Application Notes
This product is tested for ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~59 kDa corresponding to the appropriate cell lysate or extract.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 30% Glycerol
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: Histone Deacetylase 2/HDAC2
Long Name
Histone Deacetylase 2
Alternate Names
HDAC2, YAF1
Entrez Gene IDs
3066 (Human)
Gene Symbol
HDAC2
UniProt
Additional Histone Deacetylase 2/HDAC2 Products
Product Documents for Histone Deacetylase 2/HDAC2 Antibody
Product Specific Notices for Histone Deacetylase 2/HDAC2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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