Histone Deacetylase 2/HDAC2 [p Ser394] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29519

Novus Biologicals, part of Bio-Techne
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NBP2-29519
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse, Rat

Predicted:

Bovine (100%), Chicken (100%), Guinea Pig (100%), Human (100%), Primate (100%), Sheep (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Histone Deacetylase 2/HDAC2 Antibodies »

Product Summary for Histone Deacetylase 2/HDAC2 [p Ser394] Antibody

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser394 conjugated to KLH. Accession # Q92769

Modification

p Ser394

Specificity

Specific for endogenous levels of the ~55 kDa Histone Deacetylase 2/HDAC2 protein phosphorylated at Ser394. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Histone Deacetylase 2/HDAC2 [p Ser394] Antibody

Western Blot: Histone Deacetylase 2/HDAC2 [p Ser394] Antibody [NBP2-29519]

Western Blot: Histone Deacetylase 2/HDAC2 [p Ser394] Antibody [NBP2-29519]

Western Blot: HDAC2 [p Ser394] Antibody [NBP2-29519] - Western blot of mouse heart lysate showing specific immunolabeling of the ~55k HDAC2 protein phosphorylated at Ser394 (control). Phosphospecificity is shown in the second lane. The blot is identical to the control except that the lysate was incubated in lambda-Ptase (800 units/1mg protein for 30 min). The immunolabeling is completely eliminated by treatment with lambda-Ptase.

Applications for Histone Deacetylase 2/HDAC2 [p Ser394] Antibody

Application
Recommended Usage

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Histone Deacetylase 2/HDAC2

Histone Deacetylase 2 (HDAC2) is part of a family of histone deacetylases that are responsible for deacetylation of lysine residues in the histone core. HDAC2 is classified as a class I histone deacetylase and is ubiquitously expressed throughout the body (Kee et al, 2008). It has been shown that HDAC2 plays an important role in cardiac hypertrophy (Eom et al, 2011). Phosphorylation of ser394 is responsible for the hypertrophy-associated activation of HDAC2, whereas intrinsic basal activity is maintained by phosphorylation of ser422 and ser424 (EOM et al, 2011).

Long Name

Histone Deacetylase 2

Alternate Names

HDAC2, YAF1

Entrez Gene IDs

3066 (Human)

Gene Symbol

HDAC2

UniProt

Q92769 (Human)

Additional Histone Deacetylase 2/HDAC2 Products

Product Documents for Histone Deacetylase 2/HDAC2 [p Ser394] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Histone Deacetylase 2/HDAC2 [p Ser394] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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