Histone Deacetylase 4/HDAC4 Antibody (9A6E2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15463

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9A6E2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Histone Deacetylase 4/HDAC4 Antibodies »

Product Summary for Histone Deacetylase 4/HDAC4 Antibody (9A6E2)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Histone Deacetylase 4/HDAC4 (P56524). MSSQSHPDGLSGRDQPVELLNPARVNHMPSTVDVATALPLQVAPSAVPMDLRLDHQFSLPVAEPALREQQLQQELLALKQKQQIQRQILIAEFQRQHEQL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone Deacetylase 4/HDAC4 Antibody (9A6E2)

Western Blot: Histone Deacetylase 4/HDAC4 Antibody (9A6E2) [NBP3-15463]

Western Blot: Histone Deacetylase 4/HDAC4 Antibody (9A6E2) [NBP3-15463]

Western Blot: Histone Deacetylase 4/HDAC4 Antibody (9A6E2) [NBP3-15463] - Western blot analysis of extracts of various cell lines, using Histone Deacetylase 4/HDAC4 Rabbit mAb (NBP3-15463) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Applications for Histone Deacetylase 4/HDAC4 Antibody (9A6E2)

Application
Recommended Usage

Western Blot

1:100 - 1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Histone Deacetylase 4/HDAC4

Chromatin is a highly specialized structure composed of tightly compacted chromosomal DNA. Gene expression within the nucleus is controlled, in part, by a host of protein complexes which continuously pack and unpack the chromosomal DNA. One of the known mechanisms of this packing and unpacking process involves the acetylation and deacetylation of the histone proteins comprising the nucleosomal core. Acetylated histone proteins confer accessibility of the DNA template to the transcriptional machinery for expression. Histone deacetylases (HDACs) are chromatin remodeling factors that deacetylate histone proteins and thus, may act as transcriptional repressors. HDACs are classified by their sequence homology to the yeast HDACs and there are currently 2 classes. Class I proteins are related to Rpd3 and members of class II resemble Hda1p.

Long Name

Histone Deacetylase 4

Alternate Names

HDAC4

Gene Symbol

HDAC4

Additional Histone Deacetylase 4/HDAC4 Products

Product Documents for Histone Deacetylase 4/HDAC4 Antibody (9A6E2)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Histone Deacetylase 4/HDAC4 Antibody (9A6E2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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