Histone Deacetylase 4/HDAC4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16793

Novus Biologicals, part of Bio-Techne
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NBP2-16793
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Histone Deacetylase 4/HDAC4 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Histone Deacetylase 4/HDAC4. The exact sequence is proprietary.

Reactivity Notes

Chicken (85%).

Localization

Nucleus; Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

119 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Histone Deacetylase 4/HDAC4 Antibody

Western Blot: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793]

Western Blot: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793]

Western Blot: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Histone Deacetylase 4/HDAC4 Antibody, Internal (NBP2-16793) diluted at 1:1000. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793]

Immunocytochemistry/ Immunofluorescence: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793]

Immunocytochemistry/Immunofluorescence: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using HDAC4 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.
Immunohistochemistry-Paraffin: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793]

Immunohistochemistry-Paraffin: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793]

Immunohistochemistry-Paraffin: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793] - Human colon carcinoma, using HDAC4 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
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Applications for Histone Deacetylase 4/HDAC4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000
Application Notes
IHC-FR-Assay dependent

Reviewed Applications

Read 1 review rated 5 using NBP2-16793 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Histone Deacetylase 4/HDAC4

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3. [provided by RefSeq]

Long Name

Histone Deacetylase 4

Alternate Names

HDAC4

Gene Symbol

HDAC4

UniProt

P56524 (Human)

Additional Histone Deacetylase 4/HDAC4 Products

Product Documents for Histone Deacetylase 4/HDAC4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Histone Deacetylase 4/HDAC4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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