Histone Deacetylase 4/HDAC4 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22151
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Primate
Cited:
Human, Mouse, Transgenic Mouse
Predicted:
Chicken (94%), Zebrafish (92%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot
Cited:
IF/IHC, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A synthetic peptide made to a C-terminal portion of the human HDAC4 protein (between residues 1000-1084). [UniProt P56524]
Localization
Nucleus and cytoplasm.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
130 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Histone Deacetylase 4/HDAC4 Antibody - BSA Free
Western Blot: Histone Deacetylase 4/HDAC4 AntibodyBSA Free [NBP2-22151]
Western Blot: Histone Deacetylase 4/HDAC4 Antibody [NBP2-22151] - Expression of HDACs in murine optic nerve. Western blot analysis of HDAC proteins in E16, P5 and P30 murine optic nerve was performed to check the specificity of the antibodies used for the study. Blots were normalized to beta-TUBULIN. Densitometric graphs for each HDAC panel show quantification of protein levels expressed as fold-change from E16. p < 0.05 was considered statistically significant and represented by a single *, whereas p < 0.005 is represented by **. Bars show mean +/- SEM of three samples per timepoint. Note that the apparent increase in band intensities for some P5 samples reflect increased protein loading in those lanes. Image collected and cropped by CiteAb from the following publication (https://bmcdevbiol.biomedcentral.com/articles/10.1186/1471-213X-14-30), licensed under a CC-BY license.Immunocytochemistry/ Immunofluorescence: Histone Deacetylase 4/HDAC4 Antibody - BSA Free [NBP2-22151]
Immunocytochemistry/Immunofluorescence: Histone Deacetylase 4/HDAC4 Antibody [NBP2-22151] - HDAC4 antibody was tested in HeLa cells at a 1:100 dilution against DyLight 488 (Green) using a 40X objective. Alpha tubulin and nuclei were counterstained against DyLight 568 (Red) and DAPI (Blue), respectively.Immunohistochemistry: Histone Deacetylase 4/HDAC4 Antibody - BSA Free [NBP2-22151]
Immunohistochemistry: Histone Deacetylase 4/HDAC4 Antibody [NBP2-22151] - HDAC4 localization pattern in developing murine optic nerve. Optic nerve sections at P30 were triple-labeled with HDAC4 (red). Double-label of SOX2 and HDAC4. Composite image in M show z stacks of optic nerve at high magnification; horizontal yellow lines correspond the x axis plane and vertical yellow lines corresponds to the y axis. The x, z axis is shown at the bottom of each panel, while the y, z axis is shown to the right of the panel. Abbreviations: R; retina, ON; optic nerve. Scale bars = 50 um. Image collected and cropped by CiteAb from the following publication (https://bmcdevbiol.biomedcentral.com/articles/10.1186/1471-213X-14-30), licensed under a CC-BY license.Applications for Histone Deacetylase 4/HDAC4 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:500 - 1:1000
Immunohistochemistry
1:300
Immunohistochemistry-Paraffin
1:300
Simple Western
1:100
Western Blot
1.0 ug/mL
Application Notes
This HDAC4 antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry on paraffin embedded sections. In Western blot a band was observed approx. 130 kDa. In ICC/IF nuclear and cytoplasmic staining was observed in HeLa cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Histone Deacetylase 4/HDAC4
Long Name
Histone Deacetylase 4
Alternate Names
HDAC4
Gene Symbol
HDAC4
UniProt
Additional Histone Deacetylase 4/HDAC4 Products
Product Documents for Histone Deacetylase 4/HDAC4 Antibody - BSA Free
Product Specific Notices for Histone Deacetylase 4/HDAC4 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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