Histone H1 Antibody (HH1/957) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47754

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # HH1/957

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all Histone H1 Antibodies »

Product Summary for Histone H1 Antibody (HH1/957) - Azide and BSA Free

Immunogen

Recombinant full-length human H1F0 Histone H1.0 protein

Localization

Nuclear

Marker

Pan Nuclear Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-45184).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Histone H1 Antibody (HH1/957) - Azide and BSA Free

Western Blot: Histone H1 Antibody (HH1/957)Azide and BSA Free [NBP2-47754]

Western Blot: Histone H1 Antibody (HH1/957)Azide and BSA Free [NBP2-47754]

Western Blot: Histone H1 Antibody (HH1/957) - Azide and BSA Free [NBP2-47754] - Western Blot Analysis of human heart tissue lysate using Histone H1 Antibody (HH1/957).
Immunocytochemistry/ Immunofluorescence: Histone H1 Antibody (HH1/957) - Azide and BSA Free [NBP2-47754]

Immunocytochemistry/ Immunofluorescence: Histone H1 Antibody (HH1/957) - Azide and BSA Free [NBP2-47754]

Immunocytochemistry/Immunofluorescence: Histone H1 Antibody (HH1/957) - Azide and BSA Free [NBP2-47754] - Human cell culture cells were fixed with paraformaldehyde, permeabilized and incubated 1 hour at RT with primary antibody. This image was submitted via customer review.
Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) - Azide and BSA Free [NBP2-47754]

Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) - Azide and BSA Free [NBP2-47754]

Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) - Azide and BSA Free [NBP2-47754] - Formalin-fixed, paraffin-embedded human Pancreas stained with Histone H1 Antibody (HH1/957).
View 8 more images

Applications for Histone H1 Antibody (HH1/957) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5 - 1 ug/million cells in 0.1 ml

Immunofluorescence

0.5 - 1.0 ug/ml

Immunohistochemistry-Paraffin

0.5 - 1.0 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

1mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Histone H1

Histone H1 is the linker protein that is 194 amino acids long, weights approximately 21 kDa, and is part of a group of basic nuclear proteins called histones, which are responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Histone H1 has a shorter isoform measuring 177 amino acids long and weighing approximately 19 kDa as well. Current studies are being done on several diseases and disorders including paratyphoid fever, hemochromatosis, breast cancer, embryonal carcinoma, prostatitis, carcinoma, and neuronitis. This protein has also been shown to have interactions with ADPRH, CDK2, CDK7, CTCFL, and DOT1L in pathways such as the histone modification, DNA replication, chromosome condensation, PKA signaling, granzyme-A, and apoptosis pathways.

Alternate Names

H1 histone family, member 1, H1.1, H1A, H1F1HIST1, histone 1, H1a, histone cluster 1, H1a, histone H1.1, MGC126642, MGC138345

Gene Symbol

H1-1

Additional Histone H1 Products

Product Documents for Histone H1 Antibody (HH1/957) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Histone H1 Antibody (HH1/957) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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