Histone H1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-748
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Mammal
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Calf thymus intact Histone H1 (MW 23 kDa) complexed to rRNA.
Reactivity Notes
Cross-reacts with all Mammals.
Localization
Nuclear
Specificity
This specifically binds to the Histone H1 protein found in nucleosomes of all eukaryotic cells. Differences between H1 subfractions of one species are greater than the differences across species. No cross reactivity was observed with other Histone core proteins H2A, H2B, H3 or H4.
Marker
Nuclear Marker
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Histone H1 Antibody
![Western Blot: Histone H1 Antibody [NB100-748]](https://resources.bio-techne.com/images/products/Histone-H1-Antibody-Western-Blot-NB100-748-img0001.jpg "Western Blot: Histone H1 Antibody [NB100-748]")
Western Blot: Histone H1 Antibody [NB100-748]
Western Blot: Histone H1 Antibody [NB100-748] - The blot shows the antibody reactiving against porcine Histone H1. (Lane 1: negative control). Zhu G et al. j. Biol. Chem. 2005, 280:23057-23065Applications for Histone H1 Antibody
Application
Recommended Usage
Immunohistochemistry
1:100
Immunohistochemistry-Paraffin
1:100
Immunoprecipitation
1:20
Western Blot
1:300
Application Notes
Optimal dilutions/concentrations should be determined by the end user.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Histone H1
Alternate Names
H1 histone family, member 1, H1.1, H1A, H1F1HIST1, histone 1, H1a, histone cluster 1, H1a, histone H1.1, MGC126642, MGC138345
Gene Symbol
H1-1
Additional Histone H1 Products
Product Documents for Histone H1 Antibody
Product Specific Notices for Histone H1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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