Histone H1.4 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16044

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Histone H1.4 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Histone H14 (NP_005312.1). MSETAPAAPAAPAPAEKTPVKKKARKSAGAAKRKASGPPVSELITKAVAASKERSGVSLAALKKALAAAGYDVEKNNSRIKLGLKSLVSKGTLVQTKGTG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone H1.4 Antibody - Azide and BSA Free

Western Blot: Histone H1.4 AntibodyAzide and BSA Free [NBP3-16044]

Western Blot: Histone H1.4 AntibodyAzide and BSA Free [NBP3-16044]

Western Blot: Histone H1.4 Antibody [NBP3-16044] - Western blot analysis of extracts of various cell lines, using Histone H1.4 antibody (NBP3-16044) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Applications for Histone H1.4 Antibody - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 50% glycerol, pH7.3

Format

Azide and BSA Free

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Histone H1.4

The HIST1H1E gene encodes a 219 amino acid long, 21 kDA Histone H1.4 that functions by binding to linker DNA between nucleosome creating the macromolecular structure chromatin fiber. H1 histones as especially critical in condensation of nucleosome chains and the monitoring of individual gene transcription. HIST1H1E participates in PKA signaling, cell cycle start of DNA replication in early S phase, apoptosis, and histone modification. It interacts with genes HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, and HIST1H4L. HIST1H1E has been researched regarding its role in progressive supranuclear palsy, pancreatitis, hepatitis, and x inactivation.

Alternate Names

dJ221C16.5, H1 histone family, member 4, H1.4, H1F4H1E, histone 1, H1e, histone cluster 1, H1e, histone H1.4, Histone H1b, MGC116819

Gene Symbol

H1-4

Additional Histone H1.4 Products

Product Documents for Histone H1.4 Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Histone H1.4 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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