Histone H2B [ubiquitylated Lys123] Antibody (1B3F12/A9) [Janelia Fluor® 549]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54770JF549

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NBP2-54770AF350

Alexa Fluor 405

NBP2-54770AF405

Alexa Fluor 488

NBP2-54770AF488

Alexa Fluor 532

NBP2-54770AF532

Alexa Fluor 594

NBP2-54770AF594

Alexa Fluor 647

NBP2-54770AF647

Alexa Fluor 700

NBP2-54770AF700

Alexa Fluor 750

NBP2-54770AF750

Allophycocyanin

NBP2-54770APC

Biotin

NBP2-54770B

CoraFluor 1

NBP2-54770CL1

DyLight 350

NBP2-54770UV

DyLight 405

NBP2-54770V

DyLight 488

NBP2-54770G

DyLight 550

NBP2-54770R

DyLight 594

NBP2-54770DL594

DyLight 650

NBP2-54770C

DyLight 680

NBP2-54770FR

DyLight 755

NBP2-54770IR

FITC

NBP2-54770F

HRP

NBP2-54770H

Janelia Fluor 525

NBP2-54770JF525

Janelia Fluor 585

NBP2-54770JF585

Janelia Fluor 635

NBP2-54770JF635

Janelia Fluor 646

NBP2-54770JF646

Janelia Fluor 669

NBP2-54770JF669

mFluor Violet 450 SE

NBP2-54770MFV450

mFluor Violet 500 SE

NBP2-54770MFV500

mFluor Violet 610 SE

NBP2-54770MFV610

PE

NBP2-54770PE

PerCP

NBP2-54770PCP

Forumulation

Catalog #

BSA Free

NBP2-54770

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, S. pombe, Yeast

Applications

Chromatin Immunoprecipitation (ChIP), ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Janelia Fluor 549

Antibody Source

Monoclonal Mouse IgG₁ Clone # 1B3F12/A9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Histone H2B Antibodies »

Product Specifications

Immunogen

Ub-polypeptide antigen based on amino acids 115 - 130 of yeast Histone H2B

Reactivity Notes

Saccharomyces cerevisiae

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Description

Clone formerly called "H2BK123"

Applications

Application

Recommended Usage

Chromatin Immunoprecipitation (ChIP)

Optimal dilutions of this antibody should be experimentally determined.

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined. Positive control(s): Yeast histone preparation.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Histone H2B

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility tothe cellular machineries which require DNA as a template. Histones thereby play a central role in transcriptionregulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set ofpost-translational modifications of histones, also called histone code, and nucleosome remodeling

Long Name

H2B Histone Family

Alternate Names

HIST1H2BK

Gene Symbol

H2BC18

Additional Histone H2B Products

Product Documents

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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