Histone H2B [ubiquitylated Lys123] Antibody (C.15200232)

Western Blot: Histone H2B [ubiquitylated Lys123] Antibody (C.15200232) [NBP2-59149] - Histone H2B Antibody (C.15200232) [NBP2-59149] - Analysis on whole cell extracts from WT yeast cells (lane 1), yeast cells with higher steady state levels of H2BK123ub (lane 2), none ubiquitinylated H2B (lane 3) and ubiquitinylated H2A (lane 4). The position of the protein of interest is indicated on the right.

Chromatin Immunoprecipitation: Histone H2B [ubiquitylated Lys123] Antibody (C.15200232) [NBP2-59149] - Histone H2B Antibody (C.15200232) [NBP2-59149] - ChIP assays were performed using human HeLa cells, the antibody consisting of 0.5, 1, 2 and 5 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for the coding regions of the active CCT5 and EIF2S3 genes, used as positive controls, and for the inactive TSH2B gene and the Sat2 satellite repeat region used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Western Blot: Histone H2B [ubiquitylated Lys123] Antibody (C.15200232) [NBP2-59149] - Histone H2B Antibody (C.15200232) [NBP2-59149] - Analysis on histone extracts from HeLa cells (15 ug) using the antibody against H2BK123ub. The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.