Histone H2B Antibody (SD20-63)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67753

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SD20-63

Concentration

1 mg/ml

View all Histone H2B Antibodies »

Product Specifications

Immunogen

Synthetic peptide within human Histone H2B aa 110 to the C-terminus (acetyl K121). (SwissProt: O60814 Human; SwissProt: P10854 Mouse; SwissProt: Q64475 Mouse; SwissProt: Q00729 Rat)

Localization

Nucleus, Chromosome.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone H2B Antibody (SD20-63)

Western Blot: Histone H2B Antibody (SD20-63) [NBP2-67753]

Western Blot: Histone H2B Antibody (SD20-63) [NBP2-67753]

Western Blot: Histone H2B Antibody (SD20-63) [NBP2-67753] - Analysis of Histone H2B on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: Hela cell lysateLane 2: PC-12 cell lysate
Immunocytochemistry/ Immunofluorescence: Histone H2B Antibody (SD20-63) [NBP2-67753]

Immunocytochemistry/ Immunofluorescence: Histone H2B Antibody (SD20-63) [NBP2-67753]

Immunocytochemistry/Immunofluorescence: Histone H2B Antibody (SD20-63) [NBP2-67753] - Staining Histone H2B in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Histone H2B Antibody (SD20-63) [NBP2-67753]

Immunohistochemistry-Paraffin: Histone H2B Antibody (SD20-63) [NBP2-67753]

Immunohistochemistry-Paraffin: Histone H2B Antibody (SD20-63) [NBP2-67753] - Analysis of paraffin-embedded mouse testis tissue using anti-Histone H2B antibody. Counter stained with hematoxylin.
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Applications for Histone H2B Antibody (SD20-63)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:50-1:5000

Western Blot

1:500-1:2000
Application Notes
IP: Use at an assay dependent concentration.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone H2B

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility tothe cellular machineries which require DNA as a template. Histones thereby play a central role in transcriptionregulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set ofpost-translational modifications of histones, also called histone code, and nucleosome remodeling

Long Name

H2B Histone Family

Alternate Names

HIST1H2BK

Gene Symbol

H2BC18

Additional Histone H2B Products

Product Documents for Histone H2B Antibody (SD20-63)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Histone H2B Antibody (SD20-63)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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