Histone H2B [p Thr129] Antibody (SR1184)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21527

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-21527-100ul
NBP3-21527-50ul
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Yeast

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1184

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Histone H2B Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from yeast phospho-Histone H2B [Thr129] (Uniprot #: P02293)

Modification

p Thr 129

Specificity

Detects endogenous levels of total phospho-Histone H2B [Thr129]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Histone H2B [p Thr129] Antibody (SR1184)

Western Blot: Histone H2B [p Thr 129] Antibody (SR1184) [NBP3-21527] -

Western Blot: Histone H2B [p Thr 129] Antibody (SR1184) [NBP3-21527] - Western blot analysis using NBP3-21527 in Saccharomyces cerevisiae cell lysate treated with Methyl methanesulfonate.

Applications for Histone H2B [p Thr129] Antibody (SR1184)

Application
Recommended Usage

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone H2B

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility tothe cellular machineries which require DNA as a template. Histones thereby play a central role in transcriptionregulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set ofpost-translational modifications of histones, also called histone code, and nucleosome remodeling

Long Name

H2B Histone Family

Alternate Names

HIST1H2BK

Gene Symbol

H2BC18

Additional Histone H2B Products

Product Documents for Histone H2B [p Thr129] Antibody (SR1184)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Histone H2B [p Thr129] Antibody (SR1184)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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