Histone H2B, member S [p Ser14] Antibody (C.15200186)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62794

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Dot Blot, ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # C.15200186

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Histone H2B, member S Antibodies »

Product Specifications

Immunogen

Histone H2B containing the phosphorylated Serine 14 (H2BS14p)

Modification

p Ser14

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Histone H2B, member S [p Ser14] Antibody (C.15200186)

Western Blot: Histone H2B, member S [p Ser14] Antibody (C.15200186) [NBP2-62794] - HeLa cells were treated with colcemid to block the cell cycle in metaphase and histone extracts of these cells were analysed using the antibody against H2BS14p.
Dot Blot: Histone H2B, member S [p Ser14] Antibody (C.15200186) [NBP2-62794] - To test the cross reactivity of the antibody against H2BS14p, an analysis was performed with peptides containing other histone phosphorylations and the unmodified H2BS14. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. This figure shows a high specificity of the antibody for the modification of interest.
ELISA: Histone H2B, member S [p Ser14] Antibody (C.15200186) [NBP2-62794] - To determine the titer of the antibody, analysis was performed using a serial dilution of the antibody against H2BS14p.The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:2,000.

Applications for Histone H2B, member S [p Ser14] Antibody (C.15200186)

Application
Recommended Usage

Dot Blot

1:2000

ELISA

1:100

Western Blot

1:200

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.05% Sodium Azide and 0.05% ProClin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Histone H2B, member S

Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H2B is primarily acetylated at lysines 5, 12, 15, and 20 (3). Ubiquitylation of Histone H2B at lysine 123 by Rad6 has been implicated in transcriptional activation and lysine methylation on histone H3 (4). Histone H2B phosphorylation at Ser10 (for yeast) and Ser14 (for mammalian cells) has been linked to regulation of apoptosis and meiosis (5).

Alternate Names

H2B histone family S member10H2B/s, H2B histone family, member S, Histone H2B.s

Gene Symbol

H2BFS

Additional Histone H2B, member S Products

Product Documents for Histone H2B, member S [p Ser14] Antibody (C.15200186)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Histone H2B, member S [p Ser14] Antibody (C.15200186)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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