Histone H2B type 1B Antibody (SR1212)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21687

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-21687-100ul
NBP3-21687-50ul
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1212

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Histone H2B type 1B Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Histone H2B (Uniprot #: P33778)

Specificity

Detects endogenous levels of Histone H2B type 1B

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Histone H2B type 1B Antibody (SR1212)

Immunohistochemistry: Histone H2B type 1B Antibody (SR1212) [NBP3-21687] -

Immunohistochemistry: Histone H2B type 1B Antibody (SR1212) [NBP3-21687] - Immunohistochemical analysis of paraffin-embedded human colon, using NBP3-21687.

Western Blot: Histone H2B type 1B Antibody (SR1212) [NBP3-21687] -

Western Blot: Histone H2B type 1B Antibody (SR1212) [NBP3-21687] - Western blot analysis using NBP3-21687 in Hela cell treated with TSA lysate.

Applications for Histone H2B type 1B Antibody (SR1212)

Application
Recommended Usage

Flow Cytometry

1:10

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:10

Western Blot

1:5000-1:20000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone H2B type 1B

The HIST1H2BB gene encodes a 126 amino acid long, 13 kDA histone H2B type 1-B that is critical in the nucleosome by functioning in transcription regulation, DNA replication and repair, as well as chromosomal stability. It participates in histone modification, apoptosis and authophagy, protein stability, RSF complex binding to the centromer, and signal transduction Activin A signaling regulation. HIST1H2BB is known to interact with genes EP300, ERCC6, DYRK1A, NFKB2, and TNFRSF1A. It has been researched regarding its role to lupus, immunodeficiency, cockayne syndrome, and hemochromatosis.

Alternate Names

H2B histone family, member F, H2B/fHistone H2B.f, H2BFFH2B.1, histone 1, H2bb, histone cluster 1, H2bb, histone H2B type 1-B, Histone H2B.1, MGC119804

Gene Symbol

H2BC3

Additional Histone H2B type 1B Products

Product Documents for Histone H2B type 1B Antibody (SR1212)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Histone H2B type 1B Antibody (SR1212)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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