Histone H2B type 1B Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23376
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse, Rat, Xenopus
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Histone H2B synthetic peptide (coupled to carrier protein) corresponds to amino acids in the C-terminus of human histone H2B. This sequence is well conserved in rat, mouse, fish, and drosophila histone H2B, and has low homology to other histone family members. [UniProt# P33778]
Specificity
The antibody detects a 15 kDa protein corresponding to the molecular mass of Histone H2B type 1B on SDS-PAGE immunoblots of human A431, Jurkat, HeLa, mouse C2C12, rat PC12, and A7r5 cells. The antibody also shows nuclear labeling in immunocytochemistry of methanol/acetone fixed cells.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for Histone H2B type 1B Antibody
Immunocytochemistry/Immunofluorescence: Histone H2B type 1B Antibody [NBP3-23376] -
Immunocytochemistry/Immunofluorescence: Histone H2B type 1B Antibody [NBP3-23376] - Immunocytochemical labeling of Histone H2B type 1B in methanol and acetone fixed rat A7r5 cells. The cells were labeled with rabbit polyclonal Histone H2B type 1B (C-terminus) antibody (NBP3-23376), then the antibody was detected using appropriate secondary antibody conjugated to DyLight(R) 594.
Western Blot: Histone H2B type 1B Antibody [NBP3-23376] -
Western Blot: Histone H2B type 1B Antibody [NBP3-23376] - Western blot analysis of human Jurkat cells treated with calyculin A (100 nM) for 30 min. (lanes 1, 3, & 5) then the blots were treated with lambda phosphatase (lanes 2, 4, & 6). The blots were probed with anti-Histone H2B type 1B (C-terminus) (lanes 1 & 2), anti-Histone H2B type 1B (Ser-36) (lanes 3 & 4), and anti-Histone H2B type 1B (a.a. 33-47) (lanes 5 & 6).Applications for Histone H2B type 1B Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:200
Western Blot
1:5000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: Histone H2B type 1B
Alternate Names
H2B histone family, member F, H2B/fHistone H2B.f, H2BFFH2B.1, histone 1, H2bb, histone cluster 1, H2bb, histone H2B type 1-B, Histone H2B.1, MGC119804
Gene Symbol
H2BC3
Additional Histone H2B type 1B Products
Product Documents for Histone H2B type 1B Antibody
Product Specific Notices for Histone H2B type 1B Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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