Histone H3 Antibody (1B1-B2) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80770

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Chromatin Immunoprecipitation (ChIP), Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Single Cell Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Kappa Clone # 1B1-B2

Format

Azide and BSA Free

Concentration

0.5 mg/ml

View all Histone H3 Antibodies »

Product Summary for Histone H3 Antibody (1B1-B2) - Azide and BSA Free

Immunogen

This Histone H3 antibody (1B1-B2) was raised against synthetic peptide corresponding to the C-terminus of human Histone H3.

Localization

Nucleus. Chromosome.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3 Kappa

Theoretical MW

15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Histone H3 Antibody (1B1-B2) - Azide and BSA Free

Western Blot: Histone H3 Antibody (1B1-B2)Azide and BSA Free [NBP2-80770]

Western Blot: Histone H3 Antibody (1B1-B2)Azide and BSA Free [NBP2-80770]

Western Blot: Histone H3 Antibody (1B1-B2) - Azide and BSA Free [NBP2-80770] - Western blot image of Anti-Histone H3 antibody (clone 1B1-B2). Whole cell protein from HeLa (lane 1), NIH-3T3 (lane 2) and PC12 (lane 3) were separated by 4-15% SDS-PAGE and transfered to PVDF membrane. The membrane was probed with purified Anti-Histone H3 antibody at 2 ug/ml and detected with an HRP conjugated anti-mouse secondary using chemiluminescence. Observed molecular weight is ~15 kDa. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: Histone H3 Antibody (1B1-B2) - Azide and BSA Free [NBP2-80770]

Immunocytochemistry/ Immunofluorescence: Histone H3 Antibody (1B1-B2) - Azide and BSA Free [NBP2-80770]

Immunocytochemistry/Immunofluorescence: Histone H3 Antibody (1B1-B2) - Azide and BSA Free [NBP2-80770] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Histone H3 (1B1-B2) NBP2-36468 at a 1:200 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.
Immunohistochemistry: Histone H3 Antibody (1B1-B2) - Azide and BSA Free [NBP2-80770]

Immunohistochemistry: Histone H3 Antibody (1B1-B2) - Azide and BSA Free [NBP2-80770]

Immunohistochemistry: Histone H3 Antibody (1B1-B2) - Azide and BSA Free [NBP2-80770] - Analysis of Histone H3 in human breast cancer using DAB with hematoxylin counterstain. Image from the standard format of this antibody.
View 3 more images

Applications for Histone H3 Antibody (1B1-B2) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200-1:500

Immunohistochemistry-Paraffin

1:200-1:500

Single Cell Western

100 ug/ml

Western Blot

1:1000. Use reported by customer review
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone H3

Chromatin is an arrangement of DNA and proteins which form chromosomes. Chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is very dynamic, where post-translational modifications can regulate DNA to be copied, transcribed, or repaired.

Histones are nuclear proteins responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Changes in chromatin structure play a large role in the regulation of transcription. The chromatin fibers are compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.

Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. Posttranslational modifications such as acetylation of core histones regulates gene expression, thus altering protein function and regulation (1). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 and have a theoretical molecular weight of 15 kDa. Acetylation at lysine 9 and 14 appears to control histone deposition, chromatin assembly and active transcription. Methylation of arginine residues within histone H3 has also been linked to transcription regulation. Histone H3 has been linked to various types of cancer as a biomarker through the aberrant expression of histone deacetylase (HDAC) enzymes and changes to chromatins (2-4).

References

1. Zhang, Y. X., Akumuo, R. C., Espana, R. A., Yan, C. X., Gao, W. J., & Li, Y. C. (2018). The histone demethylase KDM6B in the medial prefrontal cortex epigenetically regulates cocaine reward memory. Neuropharmacology, 141, 113-125. doi:10.1016/j.neuropharm.2018.08.030

2. Nandakumar, V., Hansen, N., Glenn, H. L., Han, J. H., Helland, S., Hernandez, K, ...Meldrum, D. R. (2016). Vorinostat differentially alters 3D nuclear structure of cancer and non-cancerous esophageal cells. Sci Rep, 6, 30593. doi:10.1038/srep30593

3. Zhou, M., Li, Y., Lin, S., Chen, Y., Qian, Y., Zhao, Z., & Fan, H. (2019). H3K9me3, H3K36me3, and H4K20me3 Expression Correlates with Patient Outcome in Esophageal Squamous Cell Carcinoma as Epigenetic Markers. Dig Dis Sci, 64(8), 2147-2157. doi:10.1007/s10620-019-05529-2

4. Li, Y., Guo, D., Sun, R., Chen, P., Qian, Q., & Fan, H. (2019). Methylation Patterns of Lys9 and Lys27 on Histone H3 Correlate with Patient Outcome in Gastric Cancer. Dig Dis Sci, 64(2), 439-446. doi:10.1007/s10620-018-5341-8

Alternate Names

H3F3A

Gene Symbol

H3C14

Additional Histone H3 Products

Product Documents for Histone H3 Antibody (1B1-B2) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Histone H3 Antibody (1B1-B2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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