Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [DyLight 550]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30141R
![Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [DyLight 550]](https://resources.bio-techne.com/images/products/nbp1-30141r_mouse-monoclonal-histone-h3-trimethyl-lys9-antibody-6f12-h4-dylight-550-immunocytochemistry-immunofluorescence-2382024134115..jpg "Histone H3 [Trimethyl Lys9] (6F12-H4) in MCF7 Human Cell Line.")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Porcine, C. elegans, Drosophila, Invertebrate, Mammal, Yeast
Applications
Chromatin Immunoprecipitation (ChIP), ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
DyLight 550 (Excitation = 562 nm, Emission = 576 nm)
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 6F12-H4
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This Histone H3 [Trimethyl Lys9] antibody (6F12-H4) was raised against a synthetic peptide made to an N-terminal region of Histone H3 (between amino acids 1-50). [UniProt# P84243]
Reactivity Notes
Predicted to react with most mammalian species. Invertebrate / Blattella germanica (German cockroach) reactivity reported in scientific literature (PMID: 23872316). Porcine reactivity reported in scientific literature (PMID: 25736622)
Modification
Trimethyl Lys9
Localization
Nucleus
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [DyLight 550]
Histone H3 [Trimethyl Lys9] (6F12-H4) in MCF7 Human Cell Line.
Histone H3 [Trimethyl Lys9] (6F12-H4) was detected in immersion fixed MCF7 human breast cancer cell line using Mouse anti- Histone H3 [Trimethyl Lys9] (6F12-H4) Protein G Purified Monoclonal Antibody conjugated to DyLight 550 (Catalog # NBP1-30141R) (red) at 5 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.Applications for Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [DyLight 550]
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
Optimal dilutions of this antibody should be experimentally determined.
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Histone H3
Histones are nuclear proteins responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Changes in chromatin structure play a large role in the regulation of transcription. The chromatin fibers are compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. Posttranslational modifications such as acetylation of core histones regulates gene expression, thus altering protein function and regulation (1). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 and have a theoretical molecular weight of 15 kDa. Acetylation at lysine 9 and 14 appears to control histone deposition, chromatin assembly and active transcription. Methylation of arginine residues within histone H3 has also been linked to transcription regulation. Histone H3 has been linked to various types of cancer as a biomarker through the aberrant expression of histone deacetylase (HDAC) enzymes and changes to chromatins (2-4).
References
1. Zhang, Y. X., Akumuo, R. C., Espana, R. A., Yan, C. X., Gao, W. J., & Li, Y. C. (2018). The histone demethylase KDM6B in the medial prefrontal cortex epigenetically regulates cocaine reward memory. Neuropharmacology, 141, 113-125. doi:10.1016/j.neuropharm.2018.08.030
2. Nandakumar, V., Hansen, N., Glenn, H. L., Han, J. H., Helland, S., Hernandez, K, ...Meldrum, D. R. (2016). Vorinostat differentially alters 3D nuclear structure of cancer and non-cancerous esophageal cells. Sci Rep, 6, 30593. doi:10.1038/srep30593
3. Zhou, M., Li, Y., Lin, S., Chen, Y., Qian, Y., Zhao, Z., & Fan, H. (2019). H3K9me3, H3K36me3, and H4K20me3 Expression Correlates with Patient Outcome in Esophageal Squamous Cell Carcinoma as Epigenetic Markers. Dig Dis Sci, 64(8), 2147-2157. doi:10.1007/s10620-019-05529-2
4. Li, Y., Guo, D., Sun, R., Chen, P., Qian, Q., & Fan, H. (2019). Methylation Patterns of Lys9 and Lys27 on Histone H3 Correlate with Patient Outcome in Gastric Cancer. Dig Dis Sci, 64(2), 439-446. doi:10.1007/s10620-018-5341-8
Alternate Names
H3F3A
Gene Symbol
H3C14
Additional Histone H3 Products
Product Documents for Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [DyLight 550]
Product Specific Notices for Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [DyLight 550]
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...