Histone H3 [p Ser10] Antibody (PHH3/471R)

Unconjugated

Recombinant Monoclonal Rabbit IgG Clone # PHH3/471R

0.2 mg/ml

A synthetic peptide corresponding to (ARK-pS-TGGKAPRKQLc) of Phosphohistone H3 (phospho S10) (Uniprot: P68431)

Nucleus, Chromosome

Phosphohistone H3 (PHH3) is a marker specific for cells undergoing mitosis. Serine 10 of Histone H3 is phosphorylated in association with mitotic chromatin condensation in late G2 and M phase of the cell cycle and thus, PHH3 can distinguish mitosis from apoptotic nuclei. The range of percentage PHH3 positive tumor nuclei was from 0.0 to 6.6% (median value 0.8%). Increased expression of PHH3 was significantly associated with tumor thickness (p = 0.031), presence of tumor ulceration (p =0.041) and tumor necrosis (p =0.027), but not with Clarks level of invasion. High levels of PHH3 was associated with increased mitotic count (p = 0.003) and high Ki-67 expression (p = 0.002). For central nervous system tumors, melanoma, soft tissue tumors, GIST, etc., PHH3 mAb is helpful for tumor pathological classification and prognosis.

Nuclear Marker

Monoclonal

Rabbit

IgG

15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 1 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08511)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Protein A or G purified

1 mM PBS with 0.05% BSA

0.05% Sodium Azide

0.2 mg/ml

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at 4C.