Histone H3 [Citrulline Arg17, Citrulline Arg2, Citrulline Arg8] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-57135
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat, Bacteria
Cited:
Human, Mouse, Rat, Bacteria, WB
Applications
Validated:
Chromatin Immunoprecipitation (ChIP), ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Cited:
ELISA, Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
This Histone H3 antibody was raised against synthetic peptide derived from N terminal region of Human Histone H3, containing Citrulline at position 2;8;17
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 31160588). Use in Rat reported in scientific literature (PMID:32890741). Bacteria reactivity reported in (PMID: 32329205).
Modification
Citrulline Arg17, Citrulline Arg2, Citrulline Arg8
Specificity
Reacts with human Histone H3 Citrulline at the 3 positions: 2, 8 and 17. Reacts with mono or di H3-Cit. Not tested in other species. May cross reacts with other species due to sequence homology.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images
![Immunocytochemistry/ Immunofluorescence: Histone H3 [Citrulline Arg17, Citrulline Arg2, Citrulline Arg8] Antibody [NB100-57135]](https://resources.bio-techne.com/images/products/Histone-H3-Antibody-Immunocytochemistry-Immunofluorescence-NB100-57135-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Histone H3 [Citrulline Arg17, Citrulline Arg2, Citrulline Arg8] Antibody [NB100-57135]")
Immunocytochemistry/ Immunofluorescence: Histone H3 [Citrulline Arg17, Citrulline Arg2, Citrulline Arg8] Antibody [NB100-57135]
Immunocytochemistry/Immunofluorescence: Histone H3 [Citrulline 2, Citrulline 8, Citrulline 17] Antibody [NB100-57135] - HL60 cells treated with DMSO and Calcimycin Green: (at 1:1000 Blue Hoechst)![Western Blot: Histone H3 [Citrulline Arg17, Citrulline Arg2, Citrulline Arg8] Antibody [NB100-57135]](https://resources.bio-techne.com/images/products/Histone-H3-[Citrulline-2-Citrulline-8-Citrulline-17]-Antibody-Western-Blot-NB100-57135-img0003.jpg "Western Blot: Histone H3 [Citrulline Arg17, Citrulline Arg2, Citrulline Arg8] Antibody [NB100-57135]")
Western Blot: Histone H3 [Citrulline Arg17, Citrulline Arg2, Citrulline Arg8] Antibody [NB100-57135]
Western Blot: Histone H3 [Citrulline 2, Citrulline 8, Citrulline 17] Antibody [NB100-57135] - Histone H3/Citrulline (R2,R8,R17) at 1:10000 dilution. Observed molecular weight is ~15 kDa.Applications
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
1:10-1:500
Immunocytochemistry/ Immunofluorescence
1:200 - 1:1000
Immunohistochemistry
1:200 - 1:1000
Immunohistochemistry-Paraffin
1:200 - 1:1000
Western Blot
1:5000 - 1:50000
Application Notes
Use in IHC-Frozen reported in scientific literature (PMID:32890741).
Reviewed Applications
Read 2 reviews rated 4 using NB100-57135 in the following applications:
Formulation, Preparation, and Storage
Purification
Unpurified
Reconstitution
Lyophilized powder, reconstitute with 0.2 ml distilled water.
Formulation
Antiserum
Preservative
No Preservative
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Histone H3
Histones are nuclear proteins responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Changes in chromatin structure play a large role in the regulation of transcription. The chromatin fibers are compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. Posttranslational modifications such as acetylation of core histones regulates gene expression, thus altering protein function and regulation (1). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 and have a theoretical molecular weight of 15 kDa. Acetylation at lysine 9 and 14 appears to control histone deposition, chromatin assembly and active transcription. Methylation of arginine residues within histone H3 has also been linked to transcription regulation. Histone H3 has been linked to various types of cancer as a biomarker through the aberrant expression of histone deacetylase (HDAC) enzymes and changes to chromatins (2-4).
References
1. Zhang, Y. X., Akumuo, R. C., Espana, R. A., Yan, C. X., Gao, W. J., & Li, Y. C. (2018). The histone demethylase KDM6B in the medial prefrontal cortex epigenetically regulates cocaine reward memory. Neuropharmacology, 141, 113-125. doi:10.1016/j.neuropharm.2018.08.030
2. Nandakumar, V., Hansen, N., Glenn, H. L., Han, J. H., Helland, S., Hernandez, K, ...Meldrum, D. R. (2016). Vorinostat differentially alters 3D nuclear structure of cancer and non-cancerous esophageal cells. Sci Rep, 6, 30593. doi:10.1038/srep30593
3. Zhou, M., Li, Y., Lin, S., Chen, Y., Qian, Y., Zhao, Z., & Fan, H. (2019). H3K9me3, H3K36me3, and H4K20me3 Expression Correlates with Patient Outcome in Esophageal Squamous Cell Carcinoma as Epigenetic Markers. Dig Dis Sci, 64(8), 2147-2157. doi:10.1007/s10620-019-05529-2
4. Li, Y., Guo, D., Sun, R., Chen, P., Qian, Q., & Fan, H. (2019). Methylation Patterns of Lys9 and Lys27 on Histone H3 Correlate with Patient Outcome in Gastric Cancer. Dig Dis Sci, 64(2), 439-446. doi:10.1007/s10620-018-5341-8
Additional Histone H3 Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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