Histone H3 [Dimethyl Lys4, p Thr6] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB21-1038
![Immunocytochemistry/ Immunofluorescence: Histone H3 [Dimethyl Lys4, p Thr6] Antibody [NB21-1038]](https://resources.bio-techne.com/images/products/Histone-H3-[Dimethyl-Lys4--p-Thr6]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1038-img0003.jpg "Immunocytochemistry/ Immunofluorescence: Histone H3 [Dimethyl Lys4, p Thr6] Antibody [NB21-1038]")
Key Product Details
Species Reactivity
Human
Applications
Dot Blot, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This Histone H3 [Dimethyl Lys4, p Thr6] antibody was raised against synthetic dimethylated/phosphorylated peptides surrounding Lysine 4 and Threonine 6 of human Histone H3.2 [Swiss Prot Q71DI3].
Modification
Dimethyl Lys4, p Thr6
Localization
Nucleus. Chromosome.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Histone H3 [Dimethyl Lys4, p Thr6] Antibody
Immunocytochemistry/ Immunofluorescence: Histone H3 [Dimethyl Lys4, p Thr6] Antibody [NB21-1038]
Immunocytochemistry/Immunofluorescence: Histone H3 [Dimethyl Lys4, p Thr6] Antibody [NB21-1038] - Antibody was tested at 1:10 in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Image objective 40X.
Western Blot: Histone H3 [Dimethyl Lys4, p Thr6] Antibody [NB21-1038] - Analysis of HeLa histone prep lysate (A), NIH 3T3 histone prep lysate (B), and C. elegans histone prep lysate (C) using Histone H3 [Dimethyl Lys4, p Thr6] antibody at 2 ug/mL. Observed molecular weight is ~15 kDa
Applications for Histone H3 [Dimethyl Lys4, p Thr6] Antibody
Application
Recommended Usage
Dot Blot
1:100 - 1:2000
Immunocytochemistry/ Immunofluorescence
1:10
Western Blot
1 - 2 ug/mL
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS, 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Histone H3
Histones are nuclear proteins responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Changes in chromatin structure play a large role in the regulation of transcription. The chromatin fibers are compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. Posttranslational modifications such as acetylation of core histones regulates gene expression, thus altering protein function and regulation (1). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 and have a theoretical molecular weight of 15 kDa. Acetylation at lysine 9 and 14 appears to control histone deposition, chromatin assembly and active transcription. Methylation of arginine residues within histone H3 has also been linked to transcription regulation. Histone H3 has been linked to various types of cancer as a biomarker through the aberrant expression of histone deacetylase (HDAC) enzymes and changes to chromatins (2-4).
References
1. Zhang, Y. X., Akumuo, R. C., Espana, R. A., Yan, C. X., Gao, W. J., & Li, Y. C. (2018). The histone demethylase KDM6B in the medial prefrontal cortex epigenetically regulates cocaine reward memory. Neuropharmacology, 141, 113-125. doi:10.1016/j.neuropharm.2018.08.030
2. Nandakumar, V., Hansen, N., Glenn, H. L., Han, J. H., Helland, S., Hernandez, K, ...Meldrum, D. R. (2016). Vorinostat differentially alters 3D nuclear structure of cancer and non-cancerous esophageal cells. Sci Rep, 6, 30593. doi:10.1038/srep30593
3. Zhou, M., Li, Y., Lin, S., Chen, Y., Qian, Y., Zhao, Z., & Fan, H. (2019). H3K9me3, H3K36me3, and H4K20me3 Expression Correlates with Patient Outcome in Esophageal Squamous Cell Carcinoma as Epigenetic Markers. Dig Dis Sci, 64(8), 2147-2157. doi:10.1007/s10620-019-05529-2
4. Li, Y., Guo, D., Sun, R., Chen, P., Qian, Q., & Fan, H. (2019). Methylation Patterns of Lys9 and Lys27 on Histone H3 Correlate with Patient Outcome in Gastric Cancer. Dig Dis Sci, 64(2), 439-446. doi:10.1007/s10620-018-5341-8
Alternate Names
H3F3A, H3K4Me2
Gene Symbol
H3C14
UniProt
Additional Histone H3 Products
Product Documents for Histone H3 [Dimethyl Lys4, p Thr6] Antibody
Product Specific Notices for Histone H3 [Dimethyl Lys4, p Thr6] Antibody
This antibody is currently in testing. Please contact us for availability and pre-order. Epi-Plus (Trademark) antibody production in collaboration with Rockland Immunochemicals Inc.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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