Histone H3 [p Thr6] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB21-1051
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, C. elegans
Predicted:
Chicken (100%), Drosophila (100%), Plant (100%), Rat (100%), Xenopus (100%). Backed by our 100% Guarantee.
Applications
Chromatin Immunoprecipitation (ChIP), Dot Blot, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.45 mg/ml
Product Specifications
Immunogen
This Histone H3 [p Thr6] antibody was raised against synthetic peptide surrounding Threonine 6 of human Histone H3.2 [Swiss Prot# Q71DI3].
Reactivity Notes
Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology.
Modification
p Thr6
Localization
Nucleus. Chromosome.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Histone H3 [p Thr6] Antibody
Dot Blot: Histone H3 [p Thr6] Antibody [NB21-1051] - Analysis of Histone H3 [phospho T6] using the peptides stated above in 1.0, 0.3, 0.1 and 0.03 picomoles of peptide.
Immunocytochemistry/Immunofluorescence: Histone H3 [p Thr6] Antibody [NB21-1051] - Staining of Histone H3 [phospho T6] in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).
![Western Blot: Histone H3 [p Thr6] Antibody [NB21-1051]](https://resources.bio-techne.com/images/products/Histone-H3-[p-Thr6]-Antibody-Western-Blot-NB21-1051-img0010.jpg "Western Blot: Histone H3 [p Thr6] Antibody [NB21-1051]")
Western Blot: Histone H3 [p Thr6] Antibody [NB21-1051]
Western Blot: Histone H3 [p Thr6] Antibody [NB21-1051] - Analysis of H3pt6 in C. elegans embryo lysate. Observed molecular weight is ~15 kDa.Applications for Histone H3 [p Thr6] Antibody
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
2-5 ug per million cells
Dot Blot
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
1:200
Western Blot
1:500
Application Notes
This Histone H3 pT6 antibody is useful for ChIP*, ICC/IF, Dot Blot, and Western Blot where a band is seen ~15kDa in HeLa histone prep and C. elegans embryo lysate.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
0.45 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Histone H3
Histones are nuclear proteins responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Changes in chromatin structure play a large role in the regulation of transcription. The chromatin fibers are compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. Posttranslational modifications such as acetylation of core histones regulates gene expression, thus altering protein function and regulation (1). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 and have a theoretical molecular weight of 15 kDa. Acetylation at lysine 9 and 14 appears to control histone deposition, chromatin assembly and active transcription. Methylation of arginine residues within histone H3 has also been linked to transcription regulation. Histone H3 has been linked to various types of cancer as a biomarker through the aberrant expression of histone deacetylase (HDAC) enzymes and changes to chromatins (2-4).
References
1. Zhang, Y. X., Akumuo, R. C., Espana, R. A., Yan, C. X., Gao, W. J., & Li, Y. C. (2018). The histone demethylase KDM6B in the medial prefrontal cortex epigenetically regulates cocaine reward memory. Neuropharmacology, 141, 113-125. doi:10.1016/j.neuropharm.2018.08.030
2. Nandakumar, V., Hansen, N., Glenn, H. L., Han, J. H., Helland, S., Hernandez, K, ...Meldrum, D. R. (2016). Vorinostat differentially alters 3D nuclear structure of cancer and non-cancerous esophageal cells. Sci Rep, 6, 30593. doi:10.1038/srep30593
3. Zhou, M., Li, Y., Lin, S., Chen, Y., Qian, Y., Zhao, Z., & Fan, H. (2019). H3K9me3, H3K36me3, and H4K20me3 Expression Correlates with Patient Outcome in Esophageal Squamous Cell Carcinoma as Epigenetic Markers. Dig Dis Sci, 64(8), 2147-2157. doi:10.1007/s10620-019-05529-2
4. Li, Y., Guo, D., Sun, R., Chen, P., Qian, Q., & Fan, H. (2019). Methylation Patterns of Lys9 and Lys27 on Histone H3 Correlate with Patient Outcome in Gastric Cancer. Dig Dis Sci, 64(2), 439-446. doi:10.1007/s10620-018-5341-8
Alternate Names
H3F3A, H3T6p
Gene Symbol
H3C14
UniProt
Additional Histone H3 Products
Product Documents for Histone H3 [p Thr6] Antibody
Product Specific Notices for Histone H3 [p Thr6] Antibody
Epi-Plus antibody production in collaboration with Rockland Immunochemicals Inc. *Chromatin immunoprecipitation was performed on this product with a standard set of primers that amplify active gene loci (GAPDH, RPL30), inactive loci (MYOD1, AFM), and heterochromatic loci (alphaSat, SAT2). The resulting data indicated there was little to no amplification of these sites, which corresponds with the current literature. Until a more specific locus can be established and validated, we give this product validity for ChIP and it is backed by the full Novus guarantee.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...