Histone H3 [Asym-dimethyl Arg8] Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB21-1062
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, C. elegans
Cited:
Human
Predicted:
Chicken (100%), Drosophila (100%), Plant (100%), Rat (100%), Xenopus (100%). Backed by our 100% Guarantee.
Applications
Validated:
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Chemotaxis, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
0.84 mg/ml
Product Specifications
Immunogen
This Histone H3 [Asym-dimethyl Arg8] antibody was raised against synthetic asymmetric dimethylated peptide surrounding Arginine 8 of human Histone H3.2 [Swiss Prot# Q71DI3].
Reactivity Notes
Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology.
Modification
Asym-dimethyl Arg8
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Histone H3 [Asym-dimethyl Arg8] Antibody - BSA Free
Chromatin Immunoprecipitation: Histone H3 [Asym-dimethyl Arg8] Antibody [NB21-1062] - Chromatin from one million formaldehyde cross-linked HeLa cells was used with 2ug of NB21-1062 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.
![Western Blot: Histone H3 [Asym-dimethyl Arg8] AntibodyBSA Free [NB21-1062]](https://resources.bio-techne.com/images/products/Histone-H3-[Asym-dimethyl-Arg8]-Antibody-Western-Blot-NB21-1062-img0008.jpg "Western Blot: Histone H3 [Asym-dimethyl Arg8] AntibodyBSA Free [NB21-1062]")
Western Blot: Histone H3 [Asym-dimethyl Arg8] AntibodyBSA Free [NB21-1062]
Western Blot: Histone H3 [Asym-dimethyl Arg8] Antibody [NB21-1062] - Analysis of Histone H3R8me2 in: (1) HeLa Histone Prep Lysate, (2) NIH 3T3 Histone Prep Lysate, and (3) C. elegans Embryo Lysate. Observed molecular weight is ~15 kDa.
Immunocytochemistry/Immunofluorescence: Histone H3 [Asym-dimethyl Arg8] Antibody [NB21-1062] - Immunofluorescence: [NB21-1062] - Histone H3.2 R8me2a antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Applications for Histone H3 [Asym-dimethyl Arg8] Antibody - BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
2-5 ug per million cells
Immunocytochemistry/ Immunofluorescence
1:200
Immunohistochemistry-Paraffin
reported in scientific literature (PMID 30382083)
Western Blot
1:500
Application Notes
In Western Blot, a band is seen ~15 kDa in Hela histone prep, NIH 3T3 histone prep, and C. elegans embryo lysate.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS and 30% Glycerol
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
0.84 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Histone H3
Histones are nuclear proteins responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Changes in chromatin structure play a large role in the regulation of transcription. The chromatin fibers are compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. Posttranslational modifications such as acetylation of core histones regulates gene expression, thus altering protein function and regulation (1). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 and have a theoretical molecular weight of 15 kDa. Acetylation at lysine 9 and 14 appears to control histone deposition, chromatin assembly and active transcription. Methylation of arginine residues within histone H3 has also been linked to transcription regulation. Histone H3 has been linked to various types of cancer as a biomarker through the aberrant expression of histone deacetylase (HDAC) enzymes and changes to chromatins (2-4).
References
1. Zhang, Y. X., Akumuo, R. C., Espana, R. A., Yan, C. X., Gao, W. J., & Li, Y. C. (2018). The histone demethylase KDM6B in the medial prefrontal cortex epigenetically regulates cocaine reward memory. Neuropharmacology, 141, 113-125. doi:10.1016/j.neuropharm.2018.08.030
2. Nandakumar, V., Hansen, N., Glenn, H. L., Han, J. H., Helland, S., Hernandez, K, ...Meldrum, D. R. (2016). Vorinostat differentially alters 3D nuclear structure of cancer and non-cancerous esophageal cells. Sci Rep, 6, 30593. doi:10.1038/srep30593
3. Zhou, M., Li, Y., Lin, S., Chen, Y., Qian, Y., Zhao, Z., & Fan, H. (2019). H3K9me3, H3K36me3, and H4K20me3 Expression Correlates with Patient Outcome in Esophageal Squamous Cell Carcinoma as Epigenetic Markers. Dig Dis Sci, 64(8), 2147-2157. doi:10.1007/s10620-019-05529-2
4. Li, Y., Guo, D., Sun, R., Chen, P., Qian, Q., & Fan, H. (2019). Methylation Patterns of Lys9 and Lys27 on Histone H3 Correlate with Patient Outcome in Gastric Cancer. Dig Dis Sci, 64(2), 439-446. doi:10.1007/s10620-018-5341-8
Additional Histone H3 Products
Product Documents for Histone H3 [Asym-dimethyl Arg8] Antibody - BSA Free
Product Specific Notices for Histone H3 [Asym-dimethyl Arg8] Antibody - BSA Free
Epi-Plus antibody production in collaboration with Rockland Immunochemicals Inc.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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