Histone H3 [ac Lys14, ac Lys9] Antibody - BSA Free

Western Blot: Histone H3 [ac Lys14, ac Lys9] Antibody [NBP2-59181]

Western Blot: Histone H3 [ac Lys14, ac Lys9] Antibody [NBP2-59181] - Histone extracts of HeLa cells (15 ug) were analysed by Western blot using the antibody directed against H3K9/14ac diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.

Chromatin Immunoprecipitation: Histone H3 [ac Lys14, ac Lys9] Antibody [NBP2-59181] - ChIP assays were performed using HeLa cells, the antibody against H3K9/14ac and optimized primer pairs for qPCR. ChIP was performed with a ChIP kit, using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 ug per ChIP experiment was analysed. IgG (5 ug/IP) was used as negative IP control.QPCR was performed using primers specific for the promoter of the ACTB gene as a positive control target and for exon 2 of the MB gene as a negative control target. Figure shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA). These results confirm the observation that acetylation of H3K9/14 is present at active promoters.

Chromatin Immunoprecipitation: Histone H3 [ac Lys14, ac Lys9] Antibody [NBP2-59181] - ChIP assays were performed using HeLa cells, the antibody against H3K9/14ac and optimized primer pairs for qPCR. ChIP was performed using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 ug per ChIP experiment was analysed. IgG (5 ug/IP) was used as negative IP control. QPCR was performed using primers specific for the promoter of the ACTB gene as a positive control target and for exon 2 of the MB gene as a negative control target. Figure shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA). These results confirm the observation that acetylation of H3K9/14 is present at active promoters.