Histone H3.3 Antibody (S04-5J3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14894

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-14894-25ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S04-5J3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Histone H3.3 Antibodies »

Product Summary for Histone H3.3 Antibody (S04-5J3)

Immunogen

A synthetic peptide of human Histone H3.3 (Uniprot#: P84243)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Histone H3.3 Antibody (S04-5J3)

Western Blot: Histone H3.3 Antibody (S04-5J3) [NBP3-14894]

Western Blot: Histone H3.3 Antibody (S04-5J3) [NBP3-14894]

Western Blot: Histone H3.3 Antibody (S04-5J3) [NBP3-14894] - Western blot detection of Histone H3. 3 in K562, C6, 3T3 cell lysates using Histone H3. 3 Rabbit mAb (1:1000 diluted). Predicted band size: 15kDa. Observed band size: 15kDa.
Immunohistochemistry-Paraffin: Histone H3.3 Antibody (S04-5J3) [NBP3-14894]

Immunohistochemistry-Paraffin: Histone H3.3 Antibody (S04-5J3) [NBP3-14894]

Immunohistochemistry-Paraffin: Histone H3.3 Antibody (S04-5J3) [NBP3-14894] - Immunohistochemistry of Histone H3. 3 in paraffin-embedded Human lung cancer tissue using Histone H3. 3 Rabbit mAb at dilution 1/50

Applications for Histone H3.3 Antibody (S04-5J3)

Application
Recommended Usage

Immunohistochemistry

1/200

Western Blot

1/1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone H3.3

Alternate Names

H3.3A, H3F3, H3F3A, Histone H3.3, Histone H3.3A, Histone H3.3B, MGC87782, MGC87783

Gene Symbol

H3-3A

Additional Histone H3.3 Products

Product Documents for Histone H3.3 Antibody (S04-5J3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Histone H3.3 Antibody (S04-5J3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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