Histone H3.3A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33767

Novus Biologicals, part of Bio-Techne
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NBP1-33767
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Xenopus (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Histone H3.3A Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Histone H3.3A.3. The exact sequence is proprietary.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone H3.3A Antibody

Immunocytochemistry/ Immunofluorescence: Histone H3.3A Antibody [NBP1-33767]

Immunocytochemistry/ Immunofluorescence: Histone H3.3A Antibody [NBP1-33767]

Immunocytochemistry/Immunofluorescence: Histone H3.3A Antibody [NBP1-33767] - Paraformaldehyde-fixed HeLa, using Histone H3. 3 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.
Western Blot: Histone H3.3A Antibody [NBP1-33767]

Western Blot: Histone H3.3A Antibody [NBP1-33767]

Western Blot: Histone H3.3A Antibody [NBP1-33767] - Sample (30 ug of whole cell lysate) A: IMR32 15% SDS PAGE; antibody diluted at 1:1000.
Histone H3.3A Antibody

Western Blot: Histone H3.3A Antibody [NBP1-33767] -

Western Blot: Histone H3.3A Antibody [NBP1-33767] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3.3A antibody (NBP1-33767) diluted at 1:500.

Applications for Histone H3.3A Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Histone H3.3A

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is poyadenylated, unlike most histone genes. The protein encoded is a member of the histone H3 family.

Alternate Names

H3 histone, family 3A, H3.3AH3F3H3F3B, H3.3B, histone H3.3, MGC87782, MGC87783

Entrez Gene IDs

3020 (Human); 3021 (Human)

Gene Symbol

H3-3A

Additional Histone H3.3A Products

Product Documents for Histone H3.3A Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Histone H3.3A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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