Histone H3.3B Antibody (2D7-H1)

Novus Biologicals, part of Bio-Techne | Catalog # H00003021-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Bovine, Drosophila, Xenopus

Cited:

Mouse, Frog - Xenopus (African Clawed Frog)

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Cited:

Knockout Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2D7-H1

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Histone H3.3B Antibodies »

Product Specifications

Immunogen

H3F3B (AAH17558, 1 a.a. ~ 136 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MARTKQTARKSTGGKAPRKQLATKAARKSAPSTGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSAAIGALQEASEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 23903189). Xenopus reactivity reported in scientific literature (PMID: 23102146). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

H3F3B - H3 histone, family 3B (H3.3B)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Histone H3.3B Antibody (2D7-H1)

Western Blot: Histone H3.3B Antibody (2D7-H1) [H00003021-M01]

Western Blot: Histone H3.3B Antibody (2D7-H1) [H00003021-M01]

Western Blot: Histone H3.3B Antibody (2D7-H1) [H00003021-M01] - Analysis of H3F3B expression in transfected 293T cell line by H3F3B monoclonal antibody (M01), clone 2D7-H1.Lane 1: H3F3B transfected lysate(15 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: Histone H3.3B Antibody (2D7-H1) [H00003021-M01]

Immunocytochemistry/ Immunofluorescence: Histone H3.3B Antibody (2D7-H1) [H00003021-M01]

Immunocytochemistry/Immunofluorescence: Histone H3.3B Antibody (2D7-H1) [H00003021-M01] - Analysis of monoclonal antibody to H3F3B on HeLa cell . Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: Histone H3.3B Antibody (2D7-H1) [H00003021-M01]

Immunohistochemistry-Paraffin: Histone H3.3B Antibody (2D7-H1) [H00003021-M01]

Immunohistochemistry-Paraffin: Histone H3.3B Antibody (2D7-H1) [H00003021-M01] - Analysis of monoclonal antibody to H3F3B on formalin-fixed paraffin-embedded human liver. Antibody concentration 3 ug/ml.
View 1 more images

Applications for Histone H3.3B Antibody (2D7-H1)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Knockout Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for IHC-P, IF and ELISA. Knock Out Validation was reported in scientific literature (PMID: 26159997).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Histone H3.3B

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is poyadenylated, unlike most histone genes. The protein encoded is a member of the histone H3 family.

Alternate Names

H3 histone, family 3A, H3 histone, family 3B (H3.3B), H3.3BH3.3A, H3F3, H3F3A, histone H3.3

Entrez Gene IDs

3021 (Human)

Gene Symbol

H3F3B

OMIM

601058 (Human)

UniProt

AAH17558 (Human)

Additional Histone H3.3B Products

Product Documents for Histone H3.3B Antibody (2D7-H1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Histone H3.3B Antibody (2D7-H1)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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