Histone H4 [ac Lys20] Antibody (RM205) [DyLight 594]

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26013DL594

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-26013DL594
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Key Product Details

Species Reactivity

Validated:

Human, Vertebrate

Applications

Chromatin Immunoprecipitation (ChIP), ELISA, Immunocytochemistry/ Immunofluorescence, Multiplex Immunoassay, Western Blot

Label

DyLight 594 (Excitation = 593 nm, Emission = 618 nm)

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM205

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Histone H4 Antibodies »

Product Summary for Histone H4 [ac Lys20] Antibody (RM205) [DyLight 594]

Immunogen

An acetyl-peptide corresponding to the Histone H4 [ac Lys20]

Modification

ac Lys20

Specificity

This antibody reacts to Histone H4 acetylated at Lysine 20. No cross reactivity with other acetylated Lysines in Histone H4.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Applications for Histone H4 [ac Lys20] Antibody (RM205) [DyLight 594]

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

Optimal dilutions of this antibody should be experimentally determined.

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Multiplex Immunoassay

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Histone H4

Histone proteins H3, H4, H2A, and H2B function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibers. The nucleosome is composed of an octamer containing a H3/H4 tetramer and two H2A/H2B dimers, surrounded by approximately 146 base pairs of DNA. A diverse and elaborate array of post-translational modifications including acetylation, phosphorylation, methylation, ubiquitination, and ADP-ribosylation occurs on the N-terminal tail domains of histones. Methylation of position-specific lysine residues in histone N termini is a central modification for regulating epigenetic transitions in chromatin. Each methylatable lysine residue can exist in a mono, di, or tri methylated state. Arginine resdiues can also by mono or di methylated.

Alternate Names

H4, H4/M, H4FM, H4M, HIST1H4I, HIST4H4, Histone Cluster 1 H4, Histone Cluster 1 H4i, Histone Cluster 4 H4

Gene Symbol

H4C16

Additional Histone H4 Products

Product Documents for Histone H4 [ac Lys20] Antibody (RM205) [DyLight 594]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Histone H4 [ac Lys20] Antibody (RM205) [DyLight 594]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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